Fig. 1

The workflow of “WSI Stitcher”. Thumbnail images of WSI fragments which are downsampled by 64 folds are imported for stitching. Image matting is performed to make the image background transparent to avoid occlusion between images during the stitching process. An X-ray image with cutting records is used to assist rapid stitching. Once the stitching is completed, the lossless reconstruction starts running in the background according to the affine transform information, which does not affect the annotation and measurements of the current specimen or the stitching of the next specimen