Accuracy of next-generation sequencing for molecular profiling of small specimen of lung cancer: a prospective pilot study of side-by-side comparison

Table 3 Details of overall genetic alternations and mutations of 8-major driver genes detected in biopsy and resection specimens

Patient Number	Tumor size(mm)	Surgery					Biopsy
Patient Number	Tumor size(mm)	Total No. of alternations	Percentage of tumor cells (%)	Gene	Description	AF (%)	Total No. of alternations	Percentage of tumor cells (%)	Gene	Description	AF (%)
P01	20	3	80	EGFR	p.Leu858Arg	18.96	4	60	EGFR	p.Leu858Arg	13.31
				EGFR	p.Thr790Met	17.79			EGFR	p.Thr790Met	18.57
P02	15	3	50	ROS1	CD74-ROS1	23.67	3	30	ROS1	CD74-ROS1	21.32
P03	30	33	40	ALK	p.Pro719His	12.92	44	70	ALK	p.Pro719His	16.67
P04	15	3	60	EGFR	p.Glu746_Ala750del	20.75	13	70	EGFR	p.Glu746_Ala750del	20.88
P05	5	30	80	/			28	80	/
P06	22	4	70	EGFR	p.Leu858Arg	19.15	8	70	EGFR	p.Leu858Arg	25.74
				ALK	p.Pro542Arg	13.42			ALK	p.Pro542Arg	5.06
P07	17	7	10	/			10	10	/
P08	15	3	20	EGFR	p.Glu746_Ala750del	14.22	1	50	EGFR	p.Glu746_Ala750del	8.16
P09	25	3	40	EGFR	p.Leu858Arg	21.06	4	60	EGFR	p.Leu858Arg	12.73
P10	20	6	50	EGFR	p.Glu746_Ala750del	10.58	5	70	EGFR	p.Glu746_Ala750del	7.97
P11	80	33	30	/			36	60	/
P12	11	6	50	EGFR	p.Leu747_Pro753delinsSer	8.5	5	60	EGFR	p.Leu747_Pro753delinsSer	8.16
P13	20	18	50	KRAS	p.Gly12Cys	15.52	14	60	KRAS	p.Gly12Cys	26.59
P14	12	4	5	EGFR	p.Leu747_Thr751del	16.49	1	50	EGFR	p.Leu747_Thr751del	4.57
P15	25	9	70	KRAS	p.Gly12Val	8.13	14	50	KRAS	p.Gly12Val	8.45
P16	21	5	60	EGFR	p.Pro772_His773dup	36.71	3	80	EGFR	p.Pro772_His773dup	36.44
P17	30	16	70	EGFR	p.Leu858Arg	23.4	21	50	EGFR	p.Leu858Arg	15.67

AF Allele frequency, No. Number, ALK Anaplastic lymphoma kinase, EGFR Epidermal growth factor receptor, KRAS Kirsten rat sarcoma viral oncogene, ROS-1 ROS proto-oncogene, del Deletion, dup Duplication, delins Deletion and insertion

ISSN: 1746-1596