Fig. 2
From: Two cases of spindle cell tumors with S100 and CD34 co-expression showing novel RAF1 fusions
Genomic structures of the KIF5B-RAF1 and RAF1-KIF5B fusions identified in tumor are shown based on RNA-based NGS. A, B, Breakpoints were detected in intron 8 of KIF5B and intron 3 of RAF1, separately. The predicated resultant gene fusions were between exon 16 of KIF5B and exon 8 of RAF1, and exon 7 of RAF-1 and exon 17 of KIF5B. C. Schematic representation of the KIF5B-RAF1 and RAF1-KIF5B fusion transcripts. D, RT-PCR amplicon product revealed the KIF5B -RAF1 and RAF1-KIF5B fusion transcripts were at right size (170Â bp, 221Â bp; red arrows)