Table 1 Summary of the immunohistochemistry protocol
Antibody | Antigen retrieval treatment | Supplier | Dilution | Clone |
|---|---|---|---|---|
p53 | CC1, 64 min | Roche, Switzerland | Ready-to-use | DO7 |
c-myc | autoclave | Abcam, England | 1;200 | Y69 |
Rb1 | autoclave, pH9 | BD Pharmigen, US | 1;1000 | G3-245 |
MDM2 | autoclave | Santa cruz, US | 1;1000 | SMP14 |