Fig. 1

TILs spatial phenotype and stromal TILs (sTILs) scores across the three subtypes of breast cancer. A. Representative images depicting the spatial distribution of TILs in breast cancer. Representative images of four phenotypes of spatial TILs are presented here for a. Intra-tumoral TILs, b. Peri-tumoral TILs, c. Stromal TILs and d. Desert TILs. The yellow area represents the tumor area. Red arrows indicate TILs. Blue lines are the scale bars representing 100 μm. B. Stacked bar graph representing percent number of patients across the subtypes for four phenotypes of spatial TILs. The number of patients and percentage is shown as n (%). Distribution of number of patients across the spatial phenotypes according to their subtypes is tested by 3*4 χ2 (Chi-Square) contingency test. C. Representative images depicting stromal TILs distribution. Representative images of sTILs scores are presented here for ER+ (left panel), HER2+ (middle panel) & TNBC (right panel) with sTILs scores mentioned at the left bottom. Blue lines are the scale bars representing 200 μm. D. Box plot shows the distribution of sTILs scores across ER+, HER2+ and TNBC subtypes. The horizontal line represents the median. Error bars represent 10th and 90th percentile values. The number of patient samples (n) are shown in the box plot. The distribution of the sTILs scores amongst subtypes was analyzed for statistical significance with the Kruskal Wallis test and individual comparison between two subtypes by Mann-Whitney test, using GraphPad Prism v.5. *represent p-value of < 0.05, *** represents p-value < 0.0005