p53 immunostaining pattern is a useful surrogate marker for TP53 gene mutations

Diagnostic Pathology

Table 7 p53 immunohistochemical staining pattern and TP53 mutation discordant cases

	Primary Site	Diagnosis	P53 IHC	*TP53* mutation type	Class	alteration	allele frquency	cbioportal	MDM2 amplification	Neoadjuvant treatment
group 1	Large intestine	Adenocarcinoma	NT	WT	WT	ND		negative	Not amplified	Done
	Large intestine	Adenocarcinoma	NT	WT	WT	ND		negative	Not amplified	Done
	Large intestine	Adenocarcinoma	NT	WT	WT	ND		negative	Not amplified
	Large intestine	Adenocarcinoma	OE	WT	WT	ND		negative	Not amplified
	Large intestine	Mucinous adenocarcinoma	OE	WT	WT	ND		negative	Not amplified	Done
	Large intestine	Adenocarcinoma	OE	WT	WT	ND		negative	Not amplified
	Large intestine	Adenocarcinoma	OE	WT	WT	ND		negative	Not amplified	Done
	Anus	Adenocarcinoma	OE	WT	WT	ND		negative	Not amplified	Done
	Ovary	Papillary serous carcinoma	OE	WT	WT	ND		negative	Not amplified	Done
	Brain	Glioblastoma	OE	WT	WT	ND		negative	Amplified
group 2	Large intestine	Adenocarcinoma	OE	truncation	LOF	R342*	0.23	Likely oncogenic	Not amplified
	Large intestine	Adenocarcinoma	OE	splice site	LOF	X126_splice	0.33	Likely oncogenic	Not amplified	Done
	Large intestine	Adenocarcinoma	OE	CNV loss	LOF	loss	0	unknown	Not amplified
	Large intestine	Adenocarcinoma	OE	splice site	LOF	X261_splice	0.31	Likely oncogenic	Not amplified

ISSN: 1746-1596