Fig. 4
SNHG15/ E2F1/ECE2 axis enhances DDP resistance in LUAD cells. A, B ECE2 expression in different treatment A549/CR cells assessed by qRT-PCR and Western blot. (C) Cell viability and IC50 values of different treatment A549/CR cells determined through CCK-8. D DNA damage in different treatment A549/CR cells observed through comet assay (scale bar: 20 µm). E Immunofluorescence assay was applied to evaluate the production of γH2AX in different treatment DDP-treated (3 µg/mL) A549/CR cells transfected with sh-NC and sh-SNHG15. Photographs were taken through using confocal microscopy. 1: sh-NC + oe-NC; 2: sh-SNHG15 + oe-NC; 3: sh-NC + oe-ECE2; 4: sh-SNHG15 + oe-ECE2 (scale bars: 20 µm). (*/# indicates P < 0.05)