Fig. 2

Histology of normal submandibular gland tissue. (A) HE-staining shows ductal tissue (*), serous acinar glands, and seromucous glands. (B) ΔNp63 stains myoepithelial cells in acinar tissue and basal cells in ducts (*). (C) Calponin staining confirms myoepithelial cells in the acinar tissue, while basal cells of the ducts (*) stain negative. (D) PAS staining stains mucus in the seromucous glands. In (E), the presence of acid mucins is confirmed via Alcian Blue staining. (F) KRT7 stains all epithelial cells, while (G) KRT14 stains basal and myoepithelial cells. (H) S100p is generally negative. (I) CD117 expression is low in acinar cells. (J) GFAP is not expressed. Scale bars indicate 50 μm