Fig. 10

Validation of NRG1-mediated BNST progression through the PI3K/AKT signaling pathway using PDX models. A In vivo fluorescence imaging representation of tumor growth after sciatic nerve heterotopic transplantation. B Changes in critical proteins in the PI3K/AKT signaling pathway detected by Western blot in xenografts. C Statistical graph of protein expression in Figure B. C Tumor growth curve of PDX mice. D Kaplan-Meier survival curve of PDX mice. F Representative images of Ki67 IHC staining in xenografts. Scale bar: 100 μm. Ki67 is mainly expressed in cell nuclei. G Apoptosis of cells in xenografts detected by flow cytometry. Statistical graph in the first quadrant. H Flow cytometry detected the proportion of M2 macrophages (M2 mac) and neutrophils (neu) in xenografts—statistical graph in the first quadrant. M2 mac: CD68 + CD206 + , neu: CD14 + CD66b + ; n  = 3 per group. Indicates a difference compared to PDX-NC or PDX-con, where P  < 0.05 indicates a difference compared to PDX-NC or PDX-con, P  < 0.01 indicates a difference compared to PDX-NC or PDX-con, P < 0.001, ** indicates a difference compared to PDX-NC or PDX-con, P  < 0.0001