Fig. 3
From: Report and literature review of four cases of EWSR1::NFATC2 round cell sarcoma
A, B, C Immunohistochemical staining shows diffuse expression of CD99 and NKX2.2 in all tumor cells, along with diffuse nuclear positivity for NKX3.1 (20x). D, E Two cases show focal expression of CK and EMA (20x). F FISH detection using EWSR1 probe shows red and green signals simultaneously, along with amplification of the 5' of the EWSR1 locus (red signal). G FISH detection using EWSR1::NFATC2 fusion probe shows NFATC2 as the ligand gene for EWSR1, accompanied by amplification of fusion signals