Fig. 3

Fluorescence in situ hybridization (FISH) analysis for RET gene rearrangement. The tissue sample was hybridized with a break-apart probe for RET, where the separation of red and green signals indicates a translocation involving the RET locus. An extra green signal pattern was primarily observed in 93% of tumor cells (white arrows), break-apart signals were observed in 2% of tumor cells. Original magnification, x63