Increased expression of sialic acid in cervical biopsies with squamous intraepithelial lesions
Diagnostic Pathologyvolume 5, Article number: 74 (2010)
Altered sialylation has been observed during oncogenic transformation. Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumor progression and metastases. In the cervical cancer high levels of sialic acid have been reported in the patients serum, and an increased of total sialic acid concentration has been reported for the cervical neoplasia and cervical cancer. This study investigates the changes in expression and distribution of α2,3-linked sialic acid and α2,6- linked sialic acid in low and high squamous intraepithelial lesions and in normal tissue.
Lectin histochemistry was used to examine the expression and distribution of sialic acid in different grades of cervical neoplasia. We applied Maackia amurensis lectin, which interacts with α2,3-linked sialic acid and Sambucus nigra lectin specific for α2,6-linked sialic acid.
The histochemical analysis showed that α2,3-linked sialic acid and α2,6- linked sialic acid increased in intensity and distribution in concordance with the grade of squamous intraepithelial lesion (SIL). These results are in concordance with a previous study that reports increased RNAm levels of three sialyltransferases.
These results show that the change in sialylation occurs before cancer development and may play an important role in cellular transformation. These findings provide the basis for more detailed studies of the possible role of cell surface glycoconjugates bearing sialic acid in the cellular cervix transformation.
It is well known that tumorigenesis and metastasis are frequently associated with altered structure and expression of oligosaccharides on cell surface glycoproteins and glycolipids [1–6]. Sialylated glycoconjugates expression has been shown to change during development, differentiation, and disease and oncogenic transformation. A general increase in sialylation of cell surface glycoconjugates of carcinoma cells has been detected . These changes in sialylation are related to invasion and metastasis [8–11].
Different studies in serum from patients with cervical cancer showed that sialic acid is elevated even in the early stages of carcinoma, and levels of these glycans decreased to near normal levels after radiotherapy [12, 13]. Total sialic acid concentration of cervical tissue has been analyzed. Results showed a slight elevation in benign inflammatory lesions, moderate elevation in severe dysplasia and preinvasive carcinoma and high elevation in invasive cervical carcinoma . Sialic acids are widely distributed in nature as terminal sugars of oligosaccharides attached to proteins or lipids. Sialic acids are linked to galactose via α2,3 or α2,6-linkage or linked via α2,6-linkage to galactosamine or N-acetylgalactosamine. Sialic acids may be linked to the C8 position of another sialic acid residue . The biosynthesis of sialylated oligosaccharide sequences is catalyzed by a family of enzymes named sialyltransferases . A correlation between sialic acid and sialyltransferase expression has been detected . Altered expression of sialyltransferase in different cancers is well documented [17–20]. Increased expression of ST3Gal III and ST6Gal I was correlated with poor prognosis and lymph node metastasis in cervical cancer [21, 22] In a previous study, our group investigated the sialyltransferase expression in cervical premalignant lesions and normal tissue. Increased expression of ST3Gal III, ST3Gal IV and ST6Gal I correlated with the grade of squamous intraepithelial lesion (SIL) .
In the present study we have performed lectin histochemistry in order to determine the level of expression and the distribution of α2,3 and α2,6 sialic acid in normal tissue and in low- and high-grade cervical intraepithelial lesions in order to determine the changes in sialylation at early stages of transformation in cervical neoplasia.
Materials and methods
Sambucus nigra (SNA specific for NeuAcα2,6Gal-GalNAc); Maackia amurensis (MAA, specific for NeuAcα2,3Gal); and agglutinins biotinylated were purchased from Vector (Burlingame, CA, USA) and used at a concentration of 5 μg/ml. Signal amplification was realized with the TSA biotin system kit from NEN Life Science (Boston, MA, USA).
A retrospective study was carried out using material from the tissue collection at the Clinics of Dysplasia, Hospital General Regional No. 36 and Hospital General de Zona No. 5, Metepec, Mexican Institute of Social Security. Samples of normal cervix were obtained from uterus of patients who had undergone total hysterectomy due to uterine myoma. The biopsies were fixed in 3% phosphate-buffered formaldehyde and embedded in paraffin according to a standard protocol . All samples were obtained according to the guidelines of the Human Ethics Committee of our Institution. This study involved 23 specimens classified by a pathologist according to the Bethesda System in normal, low SIL (LSIL) or high SIL (HSIL).
Paraffin sections (5 μm) were cut and placed on poly-L-lysine-coated glass slides. The sections were deparaffinized following published methods . Sections were incubated in methanol containing 0.3% H2O2 for 30 min to block the activity of endogenous peroxidase. Hereafter, every step was followed by washing three times with 0.1 M Tris-HCl (pH 7.5), 150 mM NaCl. Slides were incubated with blocking buffer (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, and 0.5% blocking reagent (supplied in kit) for 30 min at room temperature. Sections were incubated with 5 μg/ml biotin-conjugated lectins SNA and MAA for 1 h at room temperature. After this, sections were incubated with horseradish peroxidase-conjugated streptavidin for 30 min at room temperature. Biotin signal amplification was performed for 10 min at room temperature using biotinylated tyramide. Sections were incubated one more time with horseradish peroxidase-conjugated streptavidin for 30 min. Colored reaction product was obtained with 0.01% 3,3-diaminobenzidine tetrahydrochloride.
To confirm lectin specificity, enzymatic desialylation was performed by incubating tissue sections with neuraminidase from C. perfringes (50 mU/ml in 50 mM citrate pH 6.0, 0.9% NaCl, 0.1% CaCl2) for 30 min at 37°C. Negative controls of the technique were performed in samples processed without lectin. Sections were observed in an Olympus microscope (Olympus, Tokyo, Japan), and the images were processed with the Metamorph program (Universal Imaging Co., Downingtown, PA, USA).
The intensity of carbohydrate expression was semiquantitatively classified into five groups on the basis of the intensity of positive cells: 0, negative; 1, 2, 3 and 4 (very low, low, middle and high expression) and was correlated with histological diagnosis. We also evaluated the distribution of expression in the different epithelium layers. The expression was detected in the basal, parabasal, intermediate and superficial layers in order to be able to differentiate the layers or percentage of the epithelium when we could not differentiate the layers.
In the present study we analyzed a total of 23 biopsies: 9 cases of LSIL, 9 cases of HSIL and 5 biopsies with normal epithelium.
Sialic Acid Expression
In order to determine the change in expression of α2,3 and α2,6 linkage sialic acid related to neoplastic transformation of the cervical epithelium, we performed lectin histochemistry assays with MAA and SNA.
α2,3 Sialic Acid Expression
Four out of five normal biopsies showed expression of α2,3 sialic acid. The higher expression was detected in the basal layer and only two samples with very low expression were detected in the medium and superficial layers (Table 1).
In all samples from the group with LSIL, sialic acid in α2,3 linkage was detected. They showed low and medium expression in the basal layer. For the intermediate and superficial layers, the expression was restricted to three and five samples, respectively with very low or low expression (Table 1).
Sialic acid expression in α2,3 linkage was detected in all HSIL samples analyzed (8/8) with a variable intensity of expression in the basal cells (1-4). The expression increased in the medium and superficial layers that extended in most cases to 66% or the total of the epithelium. In the layers with loss of cellular differentiation, the reaction intensity was higher with respect to layers with cellular differentiation (Table 1). The general pattern of α2,3 sialic acid expression showed an increased expression and distribution in the samples with SIL with respect to normal epithelium (Figure 1).
α2,6 Sialic Acid Expression
α2,6 Sialic acid was detected in all normal samples (5/5). Only one sample showed expression in the basal layer. All samples showed expression in the medium layers with variable intensity of expression. Four out of five samples showed expression in the superficial layers (Table 2).
In all LSIL samples, α2,6 sialic acid was detected. It was distributed in the intermediate and superficial layers. Expression at the basal and intermediate layers was very low and low, with increased expression in the superficial layer (Table 2).
All samples with HSIL diagnosis showed a medium expression of α2,6 sialic acid, with a distribution in all of them in the intermediate and superficial layer (9/9). In only two samples, expression in the basal cells was not detected (Table 2).
The general pattern of α2,6 sialic acid expression showed an increase in intensity of expression and distribution in HSIL samples. α2,6 Sialic acid expression was not detected in basal cells, with one exception (Figure 2).
Glycoconjugates on the cell surface play important roles in the regulation of cell proliferation, cell adhesion, cancer metastasis, tissue differentiation and apoptosis [7, 8]. In particular, sialylation of sugar chains has been suggested to be a very important process during development, cancer evolution, and progression, and sialic acid is often responsible for tumor-associated antigenicity [25–27]. Several studies in cervical cancer have been directed to analyze the level of sialic acid in patients' serum. The results showed that the level of these sugars is increased in patients with cervical cancer and this level return to normal after therapy. The level show a decline in patients who respond well to treatment and show an increase in patients with recurrence of cancer even prior to any clinical evidence [12, 28]. The change in sialic acid expression in premalignant lesions has been scarcely studied. Roy and Chakraborty reported a slight elevation in benign lesions, moderate elevation in severe dysplasia and preinvasive carcinoma and higher elevation in invasive carcinoma of the cervix of this monosaccharide . The present study describes the change of α2,3 and α2,6 sialic acid expression in cervical intraepithelial neoplasia. An increased expression of sialic acid was detected in both types of linkages related with the grade of cervical neoplasia in most of the studied samples. α2,3 Sialic acid expression in normal samples was detected in the basal layer, increasing distribution to the intermediate layers in LSIL. In some HSIL it was detected in all layers. α2,6 Sialic acid expression was not detected in the basal layer but was detected in the medium and superficial layers and in the same way that α2,3 sialic acid in HSIL is expressed in all layers. It is important to note that there exists a co-expression of both types of sialic acid linkages in the squamous intraepithelial lesions. The increased sialylation detected may play an important role in cellular interactions, changing the structure of the epithelium and losing definition of the different layers. The change in sialic acid expression has been correlated in most studies with a change in the expression of sialyltransferases genes [7, 29, 30]. In cervical cancer, an increase in mRNA levels of sialyltransferases ST3Gal III and ST6Gal I related with invasion has been detected . A previous study from our group found an increase in the mRNA levels of the sialyltransferases ST6Gal I, ST3Gal III and ST3Gal IV in premalignant lesions of the cervix . The results obtained in the presence study may be as a result of increased expression of certain sialyltransferases genes.
Neoplastic transformation in the human cervical epithelium was accompanied by increased expression of α2,6 and α2,3 sialic acids. These results show that the change in sialylation occurs before cancer development and may play an important role in cellular transformation. The results may be useful for the development of diagnostic techniques for cervical intraepithelial neoplasia using quantitative methods such as flow cytometry.
Baldus SE, Mönig SP, Arkenau V, Hanisch FG, Schneider PM, Thiele J, Hölscher AH, Dienes HP: Correlation of MUC5AC immunoreactivity with histopathological subtypes and prognosis of gastric carcinoma. Ann Surg Oncol. 2002, 9: 887-893. 10.1007/BF02557526.
Brockhausen I, Yang J, Lehotay M, Ogata S, Itzkowitz S: Pathways of mucin O-glycosylation in normal and malignant rat colonic epithelial cells reveal a mechanism for cancer-associated Sialyl-Tn antigen expression. Biol Chem. 2001, 382: 219-232. 10.1515/BC.2001.029.
Dall'Olio F: Protein glycosylation in cancer biology: an overview. Clin Mol Pathol. 1996, 49: 126M-135M. 10.1136/mp.49.3.M126.
Dwek MV, Ross HA, Leathem AJ: Proteome and glycosylation mapping identifies post-translational modifications associated with aggressive breast cancer. Proteomics. 2001, 1: 756-762. 10.1002/1615-9861(200106)1:6<756::AID-PROT756>3.0.CO;2-X.
Fukuda M: Possible roles of tumor-associated carbohydrate antigens. Cancer Res. 1996, 56: 2237-2244.
Ugorski M, Laskowska W: Sialyl lewis(a): a tumor associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells. Acta Biochem Pol. 2002, 49: 303-311.
Wang PH: Altered glycosylation in cancer: sialic acids and sialyltransferases. J Cancer Mol. 2005, 1: 73-81.
Lin S, Kemmer W, Grigull S, Schlag PM: Cell surface α2,6 sialylation affects adhesion of breast carcinoma cells. Exp Cell Res. 2002, 276: 101-110. 10.1006/excr.2002.5521.
Seales EC, Jurado GA, Brunson BA, Wakefield JK, Frost AR, Bellis SL: Hypersialylation of beta 1 integrins, observed in colon adenocarcinoma may contribute to cancer progression by up-regulation cell motility. Cancer Res. 2005, 65: 4645-4652. 10.1158/0008-5472.CAN-04-3117.
Inagaki Y, Tang W, Guo Q, Kokudo N, Sugawara Y, Karako H, Konishi T, Nakata M, Nagawa H, Makuuchi M: Sialoglycoconjugates expression in primary colorectal cancer and metastatic lymph node tissues. Hepatogastroenterology. 2007, 54: 53-57.
Wang FL, Cui SX, Sun LP, Qu XJ, Xie YY, Zhou L, Mu YL, Tang W, Wang YS: High expression of alpha 2,3-linked sialic acid residues is associated with the metastatic potential of human gastric cancer. Cancer Detect Prev. 2009, 32: 437-443. 10.1016/j.cdp.2009.01.001.
Balasubramaniyan N, Subramanian S, Sekar N, Bhuvarahamurthy V, Govindasamy S: Diagnostic and prognostic role of plasma and urinary sialic acid in human carcinoma of uterine cervix. Biochem Mol Biol Int. 1994, 33: 617-623.
Lagana A, Martínez BP, Marino A, Fago G, Bizzarri M: Correlation of serum sialic acid fractions as markers for carcinoma of the uterine cervix. Anticancer Res. 1995, 15: 2341-2346.
Roy A, Chakraborty S: Detection cancer cervix by estimation of sialic acid. J Indian Med Assoc. 2005, 103: 589-590.
Harduin-Lepers A, Vallejo-Ruiz V, Krzewinski-Recchi MA, Samyn-Petit B, Julien S, Delannoy P: The human sialyltransferase family. Biochimie. 2001, 83: 727-737. 10.1016/S0300-9084(01)01301-3.
Kaneko Y, Yamamoto H, Kersey DS, Colley KJ, Leestma JE, Moskal JR: The expression of Galβ1,4GlcNAc alpha2,6 sialyltransferase and alpha2,6-linked sialoglycoconjugates in human brain tumors. Acta Neuropathol. 1996, 91: 284-292. 10.1007/s004010050427.
Dall'Olio F, Malagolini N, di Stefano G, Minni F, Marrano D, Serafini-Cessi F: Increased CMP-NeuAc:Gal beta 1,4GlcNAc-R alpha 2,6 sialyltransferase activity in human colorectal cancer tissues. Int J Cancer. 1989, 44: 434-439. 10.1002/ijc.2910440309.
Recchi MA, Hebbar M, Hornez L, Harduin-Lepers A, Peyrat JP, Delannoy P: Multiplex reverse transcription polymerase chain reaction assessment of sialyltransferase expression in human breast cancer. Cancer Res. 1998, 58: 4066-4070.
Gretschel S, Haensch W, Schlag PM, Kemmer W: Clinical relevance of sialyltransferases ST6Gal I and ST3Gal III in gastric cancer. Oncology. 2003, 65: 139-145. 10.1159/000072339.
Xu L, Kurusu Y, Takizawa K, Tanaka J, Matsumoto K, Taniguchi A: Transcriptional regulation of human beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line. Biochem Biophys Res Commun. 2003, 307: 1070-1074. 10.1016/S0006-291X(03)01314-7.
Wang PH, Li YF, Juang CM, Lee YR, Chao HT, Tsai YC, Yuan CC: Altered mRNA expression of sialyltransferase in squamous cell carcinomas of the cervix. Gynecol Oncol. 2001, 83: 121-127. 10.1006/gyno.2001.6358.
Wang PH, Li YF, Juang CM, Lee YR, Chao HT, Ng HT, Tsai YC, Yuan CC: Expression of sialyltransferase family members in cervix squamous cell carcinoma correlates with lymph node metastasis. Gynecol Oncol. 2002, 86: 45-52. 10.1006/gyno.2002.6714.
López-Morales D, Velázquez-Márquez N, Valenzuela O, Santos-López G, Reyes-Leyva J, Vallejo-Ruiz V: Enhanced sialyltransferases transcription in cervical intraepithelial neoplasia. Invest Clin. 2009, 50: 45-53.
Zeller R: Fixation, embedding and sectioning of tissues embryos and single cells. Current Protocols in Molecular Biology. Edited by: Ausubel FM, Brent R, Kingston RA, Moore DD, Seidman JG, Smith JA, Strohl K. 1989, New York: Greene Publishing and Wiley Interscience, 14.1.1-14.1.8..
Hakomori S, Handa K, Iwabuchi K, Yamamura S, Prinetti A: New insights in glycosphingolipid function: "glycosignaling domain," a cell surface assembly of glycosphingolipids with signal transducer molecules, involved in cell adhesion coupled with signaling. Glycobiology. 1998, 8: xi-xix.
Varki A: Biological roles of oligosaccharides: all of the theories are correct. Glycobiology. 1993, 3: 97-130. 10.1093/glycob/3.2.97.
Vallejo V, Reyes-Leyva J, Hernández J, Ramírez H, Delannoy P, Zenteno E: Differential expression of sialic acid on porcine organs during the maturation process. Comp Biochem Physiol B. 2000, 126: 415-424. 10.1016/S0305-0491(00)00213-3.
Patel PS, Rawal GN, Balar DB: Importance of serum sialic acid and lactate dehydrogenase in diagnosis and treatment monitoring of cervical cancer patients. Gynecol Oncol. 1993, 50: 294-299. 10.1006/gyno.1993.1214.
Julien S, Adriaenssens E, Ottenberg K, Furlan A, Courtand G, Vercoutter-Edouart AS, Hanisch FG, Delannoy P, Le Bourhis X: ST6GalNAc I expression in MDA-MB-231 breast cancer cells greatly modifies their O-glycosylation pattern and enhances their tumorigenicity. Glycobiology. 2006, 16: 54-64. 10.1093/glycob/cwj033.
Sewell R, Bäckström M, Dalziel M, Gschmeissner S, Karlsson H, Noll T, Gätgens J, Clausen H, Hansson GC, Burchell J, Taylor-Papadimitriou J: The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer. J Biol Chem. 2006, 281: 3586-3594. 10.1074/jbc.M511826200.
This article is part of the requirements for obtaining the degree of PhD to Dolores López Morales in the Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina, Universidad Nacional Autónoma de México. This work was supported by CONACYT (Project SALUD-2005-13918; scholarship 166975) and by Instituto Mexicano del Seguro Social (Project FP-2003/031; scholarship 99221999).
The authors declare that they have no competing interests.
DLM processed the samples, analyzed data, and reviewed literature. GSL analyzed data and reviewed manuscript. JRL performed literature review, drafted the majority of manuscript. EZ analyzed data and reviewed manuscript. VVR conceived, designed and coordinated the study and participated in data analysis and drafted the manuscript. All authors have read and approved the final manuscript.