- Case Report
- Open Access
A juvenile case of conjunctival atypical nevus
© Colarossi et al.; licensee BioMed Central Ltd. 2013
- Received: 2 April 2013
- Accepted: 9 April 2013
- Published: 22 April 2013
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2973228795724608
Melanocytic nevi are the most common tumors of the conjunctiva, accounting for 28% of all neoplastic lesions. These tumors, despite their benign behavior, share some atypical histological features with nevi found in other anatomic sites like the genital and acral regions, globally designated as nevi with site-related atypia. Moreover, in children and adolescents, rapidly growing conjunctival nevi show sometimes worrisome histological patterns in association with a prominent inflammatory infiltrate that may lead to diagnostic problems. In this paper we describe a juvenile compound nevus characterized by marked melanocytic atypia and severe inflammation, which can be considered a rare case of juvenile conjunctival atypical nevus. The final diagnosis relied on morphological and immunohistochemical characterization of the large epithelioid melanocytic cells, and on the results of FISH analysis.
- Fish Analysis
- Virtual Slide
- Melanocytic Nevus
- Melanocytic Lesion
Among conjunctival tumors, melanocytic lesions represent 53% of all excised conjunctival lesions . Nevi are the most common, found in 52% of the cases, followed by melanoma in 25%, primary acquired melanosis (PAM) in 21%, and racial melanosis in 3% . Similarly to when they occur in the skin, nevi can be congenital, if they appear at birth or within the first 6 months of life, or acquired, if they become clinically evident in the first or second decade of life as discrete, variably pigmented, slightly elevated lesions, frequently containing clear cysts. Nevi derive from a benign proliferation of melanocytes in the basal layer of the conjunctival epithelium and are classified, like on the skin, as junctional, compound, and subepithelial, the compound being the most common pattern. Subepithelial components are frequently hyper-cellular and may have cytologic atypia, but are usually associated with symmetry, demarcation from the surrounding stroma and melanocytic maturation, all hallmarks of a benign lesion with reassuring findings . Nevi are typically located in the interpalpebral bulbar conjunctiva (67-72%), especially the interpalpebral area, followed by the caruncle (15%-22%) and tarsus (0.7%). The vast majority of nevi in these sites are benign with the exception of tarsal melanocytic lesions that are frequently regarded as suspicious for malignancy. On the other hand, conjunctival melanomas typically arise in adults (median age 62 years), but rare cases of conjunctival melanoma in children have been recognized .
Here we report a case of juvenile conjunctival nevus, whose histology was uncommonly atypical, not only in the subepithelial area, but also deeply within the lesion, thus raising the suspicion of malignancy. This was an entity that has, so far, drawn little attention in the literature. Consequently, accurate morphological and immunohistochemical studies were performed to render a final diagnosis of atypical nevus, and pertinent FISH analysis gave interesting results to support that it was of a benign nature.
In this report we present a challenging case of conjunctival compound nevus in a young female patient. The atypical histological features that characterize the lesion were what made this case peculiar. The lesion could be included in the spectrum of atypical lesions associated with the concept of “site specific atypia”, but this approach is not fully convincing, in part due to the severe inflammation - like a host reaction to malignancies - that was present in our case. Moreover, we observed nests of atypical large cells at the base of the nevus that showed no maturation activity, hence, the differential diagnosis with melanoma became the main diagnostic problem. The features that could have ruled out the diagnosis of melanoma were the sharp circumscription of the lesions and the lack of mitotic activity in the substantia propria.
In general, the patient’s young age and the cystic nature of the lesions are consistent with benign behavior, so that the vast majority of these cases are diagnosed as inflamed juvenile conjunctival nevi , taking also into account that conjunctival malignant melanoma is extremely rare in the first two decades of life.
As far as our case is concerned, the rarity of cystically dilated glands, the marked atypia of the lesional cells and the focal pagetoid spread, suggesting, per se, worrisome histological findings, were in keeping with the diagnosis of atypical nevus. Ki-67 findings were not significantly increased if compared to normal conjunctiva  Immunohistochemical results concerning p16 were in line with our histological findings. p16 protein regulates the cell cycle and plays a major role in melanomagenesis, and is expressed in several types of benign melanocytic nevi . Moreover, p16 immunohistochemical expression is helpful in some cases to differentiate between childhood nodular Spitzoid melanomas and Spitz nevi . In addition, FISH analysis was performed for diagnostic purposes. In fact, in recent papers, the application of fluorescence in situ hybridisation has been demonstrated to be a useful tool for the diagnosis of ambiguous melanocytic neoplasms of the conjunctiva [9, 10] as demonstrated in skin lesions [11, 12]. Taking into account that the sensitivity of the technique is around 87%, a positive FISH result provides additional support in favor of melanoma, whereas a negative FISH result makes melanoma less likely. In our case, FISH analysis, targeting 6p25, 6q23, 11q13 and centromere 6, did not show any genetic aberration and supported our diagnosis of atypical nevus.
The interest of this particular case relies on the differential diagnosis between atypical benign nevic lesions and melanomas in the conjunctiva, using a multi-level evaluation of morphologic, immunohistochemical and in situ molecular findings.
Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
Authors thank Ms Anna Vallerugo, MA, for the English editing.
- Zembowicz A, Mandal RV, Choopong P: Melanocytic lesions of the conjunctiva. Arch Pathol Lab Med. 2010, 134: 1785-1792.PubMedGoogle Scholar
- Novais GA, Fernandes BF, Belfort RN, Castiglione E, Cheema DP, Burnier MN: Incidence of melanocytic lesions of the conjunctiva in a review of 10 675 ophthalmic specimens. Int J Surg Pathol. 2010, 18: 60-63. 10.1177/1066896908319775.View ArticlePubMedGoogle Scholar
- Hosler GA, Moresi JM, Barrett TL: Nevi with site-related atypia: a review of melanocytic nevi with atypical histologic features based on anatomic site. J Cutan Pathol. 2008, 35: 889-898. 10.1111/j.1600-0560.2008.01041.x.View ArticlePubMedGoogle Scholar
- Strempel I, Kroll P: Conjunctival malignant melanoma in children. Ophthalmologica. 1999, 213: 129-132. 10.1159/000027405.View ArticlePubMedGoogle Scholar
- Zamir E, Mechoulam H, Micera A, Levi-Schaffer F, Pe’er J: Inflamed juvenile conjunctival naevus: clinicopathological characterization. Br J Ophthalmol. 2002, 86: 28-30. 10.1136/bjo.86.1.28.PubMed CentralView ArticlePubMedGoogle Scholar
- Sebastià R, Ventura MP, Solari HP, Antecka E, Orellana ME, Burnier MN: Immunohistochemical detection of Hsp90 and Ki-67 in pterygium. Diagnostic Pathol. 2013, 8: 32-10.1186/1746-1596-8-32.View ArticleGoogle Scholar
- Sparrow LE, Eldon MJ, English DR, Heenan PJ: p16 and p21WAF1: Protein expression in melanocytic tumors by immunohistochemistry. Am J Dermatopathol. 1998, 20: 255-261. 10.1097/00000372-199806000-00006.View ArticlePubMedGoogle Scholar
- Dhaybi RA, Agoumi M, Gagne´ I, McCuaig C, Powell J, Kokta V: p16 Expression: a marker of differentiation between childhood malignant melanomas and Spitz nevi. J Am Acad Dermatol. 2011, 65: 357-363. 10.1016/j.jaad.2010.07.031.View ArticlePubMedGoogle Scholar
- Mudhar HS, Smith K, Talley P, Whitworth A, Atkey N, Rennie IG: Fluorescence in situ hybridisation (FISH) in histologically challenging conjunctival melanocytic lesions. Br J Ophthalmol. 2013, 97: 40-46. 10.1136/bjophthalmol-2012-302261.View ArticlePubMedGoogle Scholar
- Busam KJ, Fang Y, Jhanwar SC, Pulitzer MP, Marr B, Abramson DH: Distinction of conjunctival melanocytic nevi from melanomas by fluorescence in situ hybridization. J Cutan Pathol. 2010, 37: 196-203. 10.1111/j.1600-0560.2009.01488.x.View ArticlePubMedGoogle Scholar
- Gerami P, Jewell SS, Morrison LE: Fluorescence in situ hybridisation (FISH) as an ancillary diagnostic tool in the diagnosis of melanoma. Am J Surg Pathol. 2009, 33: 1146-1156. 10.1097/PAS.0b013e3181a1ef36.View ArticlePubMedGoogle Scholar
- Massi D, Cesinaro AM, Tomasini C, Paglierani M, Bettelli S, Dal Maso L, Simi L, Salvianti F, Pinzani P, Orlando C, De Giorgi V, Lukic S, Maiorana A, Santucci M, Canzonieri V: Atypical Spitzoid melanocytic tumors: a morphological, mutational, and FISH analysis. J Am Acad Dermatol. 2011, 64 (5): 919-935. 10.1016/j.jaad.2010.05.043.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.