- Case Report
- Open Access
Adult type granulosa cell tumor of the testis with a heterologous sarcomatous component: case report and review of the literature
Diagnostic Pathology volume 9, Article number: 107 (2014)
Adult testicular granulosa cell tumors are rare sex cord- stromal tumors of which only 45 have been previously reported. As compared with their ovarian counterparts, these tumors may follow a more aggressive course because the proportion of malignant cases is higher. We report here a unique case of a 78-year Caucasian with a left sided adult type granulosa cell tumor with a heterologous sarcomatous tumor component. A heterologous sarcomatous component has occasionally been observed in ovarian tumors but never in testicular granulosa cell tumors. The sarcomatous component showed a higher number of mitotic figures (1/Hpf) and a marked proliferation rate (up to 50% Ki 67 positive cells) compared with the granulosa type tumor component. CD 99 and the progesterone receptor were positive in both tumor components, inhibin and calretinin only in the granulosa cells, and pancytokeratin only in the sarcomatouse one. Key words: testis - ovary - granulosa cells - sarcoma - inhibin Runing title: testicular sarcomatous granulosa tumor.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/6959043481207016
Tumors of sex cord/gonadal stroma are exceedingly rare tumors that, in large series, account for 1.6–6% of adult testicular tumors and occur somewhat more frequently in children. Teilum used the term gonadal stroma tumor as a histogenetic and morphologic designation for tumors of both genders which derive from an undifferentiated gonadal mesenchyme. They arise in the testis as well as in the ovary. However, the incidence of the single histological types differs widely between genders. Leydig cell tumor is the most common stromal tumor of the testis, followed by Sertoli cell tumors. Whereas granulosa cell tumors are typical for the ovaries.
The adult type of granulosa cell tumor is extremely rare in the testis – 46 cases have been described to date (Table 1)[3–31]. However, in most of the reports these tumors are only the object of immunohistochemical or cytogenetical investigations; thus, the clinical data are utterly or partially missing[12, 15, 17, 31]. In contrast to the adult type, the juvenile type of granulosa cell tumor is the most frequent testicular tumor in boys younger than six months.
We observed an adult type granulosa cell tumor with a heterologous sarcomatous tumor component, a morphological feature rarely observed in ovarian granulosa cell tumors [32-34], and only in one testicular Leydig-Sertoli cell tumor to date .
A 78-year old Caucasian male was admitted to hospital due to acute neurological symptoms. Upon exploration, he reported a history of hypertension and arrhythmia. Upon physical examination, an enlarged left testicle was noted and an inguinal herniotomy scar on the left side. According to statements by the patient, the testicle had started to enlarge a year earlier. After neurological symptoms had remitted, the patient was transferred to the urological unit for further diagnostic procedures. A painless and solid scrotal mass could be palpated on the left side. The left epididymis was not discernible at palpation. The right testicle and epididymis were unremarkable. Upon ultrasonography, the mass had a diameter of 13 cm and contained cystic spaces surrounded by solid tumor structures (Figure 1). There were no enlarged inguinal or abdominal lymph nodes. Upon computed tomography of the chest and abdomen, a cyst measuring 2.5 cm was found in the right kidney, but masses raising suspicion of metastases were not detected. As a consequence of the clinical findings, inguinal semicastration of the left testis was performed. Twenty three months following surgery, the patient is alive and without signs of progressive disease.
Macroscopically the tumor was composed of solid white or brownish firm masses with multiple cystic spaces.Microscopically the tumor consisted of rather monomorphous typical granulosa cells with scanty cytoplasm and angulated, coffee-bean like, grooved nuclei (Figure 2). The cells grew mainly in solid patterns with some trabecular and gyriform areas and a few cysts lined by tumor cells containing an eosinophilic fluid (Figure 3). Some not well-differentiated microfollicular structures resembling Call-Exner bodies could be found interspersed (Figure 4). Mitotic figures were extremely rare. The tumor showed central necrotic areas. The main part of the tumor was surrounded by fibrotic tissue in which small nests of undifferentiated, spindled cells ordered in sheets were located. These cells did not exhibit any polymorphism and mitotic figures were lacking. At the border of a hyalinized area a proliferation of large, polygonal and spindled cells with many tumor giant cells were present (Figures 5 and6). The cells had a clear or slightly eosinophylic cytoplasm and markedly polymorphic nuclei with large nucleoli. Almost all mitotic figures (1/hpf) were atypical. Accordingly, the proliferation rate of these sarcomatous cells, assessed by MIB-1 antibodies, was consistently higher (focally > 50%) than that of the typical granulosa cells (<1%). Very large, spindled sarcomatous cells were also found scattered among fibrous collagen rich tissue. The sarcomatous area was infiltrated by a startling number of eosinophilic leucocytes.
Immunohistochemically, single typical granulosa cells showed a week reactivity for inhibin and a strong but patchy distributed reactivity for calretinin. Vimentin and CD 99 (Figure 7) as well as progesterone receptor (PR) were strongly expressed in the typical granulosa cells (>70%) as well in the sarcomatous one, whereas androgen- (AR) and estrogen-(ER) receptor antibodies did not show any reactivity. Interestingly, the sarcomatous tumor cells reacted strongly with pancytokeratin antibodies (AE1:AE3) (Figure 5); by way of contrast, the typical granulosa cells were completely unreactive.
Since the FOXL2 402C → G (C134W) mutation is also present in adult-type granulosa cell tumors occurring in men, although in a smaller proportion when compared with the rates reported in women, we also performed this mutation analysis. To this end, DNA of the tumor cells was extracted from serial sections (FFPE tissue) after manual microdissection using the Maxwell 16 System (Promega GmbH, Mannheim, Germany) in accordance with manufacturer’s instructions.
Mutation hotspot region containing codon 134 was amplified by PCR (primer sense: 5′- CAACTACTGGACGCTGGACC-3′; primer antisense: 5′-GTTGCCCTTCTCGAACATGTCT-3′). Sequencing analysis was performed using the PyroMark Q24 system (Qiagen, Hilden, Germany) in accordance with manufacturer’s instructions (sequencing primer: 5′- CGCTGGACCCGGCCT-3′). Detailed information about PCR and sequencing conditions is available on request. A FOXL2p.C134W mutation was nonetheless not detectable.
Adult type testicular granulosa cell tumors (GCT) are rare sex cord–stromal tumors. However, subsequent to a thorough study of the literature we were able to find 17 cases above and beyond those referred to in one of the recent literature reviews (28 cases). Fifteen out of the 45 (33%) reported cases were published after the year 2000, which does not imply that incidence actually increased, but rather, that the tumor is more frequently being recognized.
Morphological diagnosis is based primarily on the typical morphology of the granulosa cells with their coffee-bean like, angulated and grooved nuclei. Macrofollicles ought to be present, the presence of the Call Exner bodies makes correct diagnosis easier; however, they are not always found and thus, are not indispensable to diagnosis. Young and Scully require that “all or almost all” of the tumor should be composed of granulosa cells. In fact, undifferentiated Sertoli cell tumors can have a very similar cytomorphological features but form predominantly ill-defined tubular structures resembling primitive testicular tubules and do not have follicular structures. If the morphological features are ambiguous, the term “incompletely differentiated” (WHO) is preferable. The very rare fibrothecoma of the testis can be also mistaken for undifferentiated sex cord tumor since it manifests the same immunoreactivity as all other stromal tumors. This tumor, however, is composed of highly monotone spindle cells which are embedded in an acellular fibrous stroma.
To the best of our knowledge, this is the first case of GCT with heterologous sarcomatous changes observed in the testis. The sarcomatous cells did not show any differentiation, they were negative for desmin and SMA. As in many soft tissue sarcomas, the cells were also positive for pancytokeratin antibodies (AE1:AE3) in our case. With the exception of one case which showed “some evidence of rhabdomyosarcomatous differentiation”, the sarcoma cells in all other cases described in the ovaries did not show any special differentiation[33–35].
One could argue that the observed sarcomatous component originates from a concomitant germ cell tumor growing simultaneously with the granulosa tumor. Quite apart from the fact that the tumor has been entirely processed and no germ cell tumor remnant could be found, a smooth transition from the differentiated to the sarcomatous component could be observed. Moreover, the simultaneous occurrence of germ cell and stromal tumors in the unilateral testis is extremely rare without any common genetic or oncogenetic background. In contrast to the germ cells, the cells of the gonadal stroma tumors are differentiated and not pluripotent; can therefore not give rise to somatic type malignancies (carcinomas, differentiated sarcomas) as the cells of teratomas do. The somatic type malignancies arising in teratomas are well differentiated, completely identical to the tumors originally developing in other organs e.g. soft and nervous tissue, intestine etc., whereas the sarcomas described to date in stromal tumors[32–35] were all composed of undifferentiated spindle cells. It can be assumed that these tumors develop from undifferentiated gonadal stroma.
A molecular characterization of sarcomatous changes in ovarian GCT showed a down-regulation of the GCT-specific genes such as inhibin, estrogen receptor and FSH receptor. A significant up-regulation of genes with an inflammatory response was consistent with the presence “of a marked inflammatory infiltrate”, which was a striking morphological feature in our case as well. However, contrary to these findings of McNeilage et al., the PR receptor was not down-regulated in our case, but immunohistochemically strongly expressed.
Some authors claim that testicular GCT can be mistaken for lymphoma, metastatic carcinoma or even for melanoma[12, 35, 36], but lymphoma and metastatic carcinoma cells tend to spread in the interstitium between testicular tubules and usually do not form solid tumor masses.
Immunohistochemistry is obviously helpful for achieving a correct diagnosis. One must keep in mind, however, that with few exceptions all types of sex cord/gonadal stroma tumor cells show reactivity for the same antibodies. By and large, but not in all cases, the tumor cells of GCT are positive for inhibin, calretinin, vimentin and CD 99[20, 31]. According to Renshaw et al. SMA and S-100 are constantly expressed in all testicular sex cord/gonadal stroma tumors, but other authors cannot confirm these findings[20, 30]. CD 99 is consistently expressed, whereas EMA has never been detected in the different types of sex cord/gonadal stroma tumors of the testis; these antibodies are thus useful for the differential diagnosis stromal tumor vs. carcinoma metastasis[20, 31, 40]. Pancytokeratin and desmin are often but not constantly detected[12, 20, 31]. Also very useful to the diagnosis of these tumors is the detection of estrogen (ER) and progesterone receptors (PR), and in this connection PR are detected more often than ER (75% vs. 50%). PlAP immunohistochemistry is obviously the best method to distinguish the stromal from the germ cell tumors of the testes. The PR positivity in the sarcomatous part of the tumor presented here is the only evidence that these cells derive from the granulosa cells and not from the supporting stroma.
A more difficult task is the correct classification of poorly differentiated sex cord/stroma tumors, since the granulosa and Sertoli cells can have a similar appearance and can also express all of the immunohistochemical markers discussed above. Almost all Leydig and granulosa-cell tumors, but only about half of Sertoli-cell tumors, react with inhibin and calretinin antibodies. A valuable marker could be the melan-A/MART-1 (A 103) melanoma antibody, which is positive in all Leydig and in many Seroli-cell tumors, but negative in GCTs. CD 56 is constantly expressed in GCT, but has never been tested in the diagnostics of the testicular GCTs. CD56 is a regulator of growth and differentiation in ovarian folliculogenesis.
One of the major problems of histological diagnosis of testicular sex cord/stroma tumors is the prediction of malignancy. Atypical mitotic figures occur more frequently and the mitotic index is significantly higher in malignant tumors; vascular invasion, infiltrative margins, and necrosis are also associated with malignancy, but are not very reliable when used as a single criterion. The only reliable predictor of malignancy in GCT as in all other stromal tumors is a tumor diameter > 5 cm[1, 17, 31].
Due to the few cases reported, our knowledge of the biological behavior and the clinical features of this tumor is still fragmentary. The average age of the diseased men is 45 years (Range 12 – 83 years) with an accumulation (45%) of cases in the 5th and 6th decade (Table 1). In 65.5% of cases (21/32) the tumors were smaller than 2 cm (1.7 cm in median, range: 0.7 – 15 cm). Endocrine symptoms (gynecomastia, loss of libido and potency) were observed only in six cases, 26 cases did not have such symptoms and in the other cases this clinical information is missing (for references, see Table 1). In the known cases the duration of clinical signs ranged from a few months to 10 years! Nineteen case reports included a follow up: 2 patients died of disease, one of them 11 years after diagnosis[7, 17], 3 patients died of unrelated diseases[12, 20], 3 patients were alive with disease, one of them 6 years after surgery[16, 17, 23]. The longest follow-up of one of the 13 patients who were alive without evidence of disease was 14 years.
Even this small series of cases shows that testicular adult type GCTs behave in an aggressive manner in one quarter of cases. The clinical problem is that there is no specific therapy for these tumors. Various chemotherapeutic agents and regimens have been used for patients with metastatic disease with limited or no success. The “prophylactic” retroperitoneal lymphadenectomy is an option which is very successful in stage I diseases, but not in the higher stages when the lymph nodes are already affected.
In summary, we present a unique case of a testicular granulosa cell tumor of adult type with a sarcomatous component whose impact on the clinical course is unknown.
The correct classification of poorly differentiated sex cord/stroma tumors can be difficult. Adult type testicular granulosa cell tumors (GCT) are rare sex cord–stromal tumors challenging the diagnostic skills of surgical pathologists. Rarely, these tumors can display a sarcomatous component as described in the reported case.
Written informed consent was obtained from the patient for publication of this case report.
TS is Head of the Institute of Applied Pathology, Speyer (Germany).
RS is consultant for molecular diagnostic pathology, Department of Pathology, Friedrich Alexander University, Erlangen (Germany).
AH is Head of the Department of Pathology, Friedrich Alexander University, Erlangen (Germany).
SS was consultant urologist of the Department of Urology, Hetzelstift, Neustadt/Weinstrasse (Germany).
ML is Head of the Department of Urology, Hetzlestift, Neustadt (Germany).
GM is Professor Emeritus of Pathology, Medical University Innsbruck (Austria).
Mikuz G, Colecchia M: Tumor of sex Cord/Gonadal Stroma. Edited by: Mikuz G. 2007, London: Clinical Pathology of Urological Tumors.Informa.healthcare
Teilum G: Special Tumors of the Ovary, and Testis. 1976, Copenhagen: Munks- gard, 2
Laskowski J: Feminizing tumor of the testis: general review with case report of granulosa cell tumor of the testis. Endokrynol Pol. 1952, 3: 337-343.
Cohen J, Diamond J: Leontiasis ossea, slipped epiphyses and granulosa cell tumor of the testis with renal disease: report of a case with autopsy findings. Arch Pathol. 1953, 56: 488-500.
Case record of the Massachusetts General Hospital (Case no. 41471). N Engl J Med. 1955, 253: 926-931.
Melicow MM: Classification of tumors of the testis: a clinical pathological study based on 105 primary and 13 secondary cases in adults and 3 primary and 4 secondary cases in children. J Urol. 1955, 73: 547-574.
Mostofi FK, Theiss EA, Ashley DJB: Tumors of the specialized gonadal stroma in human male patients: androblastoma, Sertoli cell tumor, granulosa theca cell tumors of the testis, and gonadal stromal tumor. Cancer. 1959, 12: 944-957.
Marshall FF, Kerr WS, Kliman B, Scully RE: Sex cord-stromal (gonadal stromal) tumors of the testis: a report of 5 cases. J Urol. 1977, 117: 180-184.
Talerman A: Pure granulosa cell tumour of the testis. Report of a case and review of the literature. Appl Pathol. 1985, 3: 117-122.
Lawrence WD, Young RH, Scully RE: Sex Cord Stromal Tumors. Edited by: Talerman A, Roth LM. 1986, Churchill Livingston: Pathology of the testis and its adnexa
Gaylis FD, August C, Yeldandi A, Nemcek A, Garnett J: Granulosa cell tumor of the adult testis: ultrastructural and ultrasonographic characteristics. J Urol. 1989, 141: 126-127.
Düe W, Dieckmann KP, Niedobitek G, Bornhöft G, Loy V, Stein H: Testicular sex cord stromal tumour with granulosa cell differentiation: detection of steroid hormone receptors as a possible basis for tumour development and therapeutic management. J Clin Pathol. 1990, 43: 732-737.
Nistal M, Lazaro R, Garcia J, Paniagua R: Testicular granulosa cell tumors of the adult type. Arch Pathol Lab Med. 1992, 16: 284-287.
Matoska J, Ondrus D, Talerman A: Malignant granulosa cell tumor of the test is associated with gynecomastia and long survival. Cancer. 1992, 69: 1769-1772.
Sasano H, Nakashima N, Matsuzaki O, Kato H, Aizawa S, Sasano N, Nagura H: Testicular sex cord-stromal lesions: immunohistochemical analysis of cytokeratin, vimentin and steroidogenic enzymes. Virchows Arch A Pathol Anat Histopathol. 1992, 421: 163-169.
Monobe Y, Manabe T: Malignant sex-cord stromal tumor of the testis: report of a case with special reference to its unusual intracytoplasmic substructures. Jpn J Clin Oncol. 1992, 22: 414-420.
Jimenez-Quintero LP, Ro JY, Zavala-Pompa A, Amin MB, Tetu B, Ordoñez NG, Ayala AG: Granulosa cell tumor of the adult testis: a clinicopathologic study of seven cases and a review of the literature. Hum Pathol. 1993, 24: 1120-1225.
Renshaw AA, Gordon M, Corless CL: Immunohistochemistry of unclassified sex cord-stromal tumors of the testis with a predominance of spindle cells. Mod Pathol. 1997, 10: 693-700.
Morgan DR, Brame KG: Granulosa cell tumour of the test is displaying immunoreactivity for inhibin. BJU Int. 1999, 83: 731-732.
Al-Bozom IA, El-Faqih SR, Hassan SH, El-Tiraifi AE, Talic RF: Granulosa cell tumor of the adult type: a case report and review of the literature of a very rare testicular tumor. Arch Pathol Lab Med. 2000, 124: 1525-1528.
Wang BY, Rabinowitz DS, Granato RC, Unger PD: Gonadal tumor with granulosa cell tumor features in an adult testis. Ann Diagn Pathol. 2002, 6: 56-60.
Guzzo T, Gerstein M, Mydlo JH: Granulosa cell tumor of the contralateral testis in a man with a history of cryptorchism. Urol Int. 2004, 72: 85-87.
Suppiah A, Musa MM, Morgan DR, North AD: Adult granulosa cell tumour of the testis and bony metastasis. A report of the first case of granulosa cell tumour of the testicle metastasising to bone. Urol Int. 2005, 75: 91-93.
Hisano M, Souza FM, Malheiros DM, Pompeo AC, Lucon AM: Granulosa cell tumor of the adult testis: report of a case and review of the literature. Clinics (Sao Paulo). 2006, 61: 77-78.
Arzola J, Hutton RL, Baughman SM, Mora RV: Adult-type testicular granulosa cell tumor: case report and review of the literature. Urology. 2006, 68: 1121.e13-6-
López JI: Adult-type granulosa cell tumor of the testis. Report of a case. Tumori. 2007, 93: 223-224.
Ditonno P, Lucarelli G, Battaglia M, Mancini V, Palazzo S, Trabucco S, Bettocchi C, Paolo SF: Testicular granulosa cell tumor of adult type: a new case and a review of the literature. Urol Oncol. 2007, 25: 322-325.
Gupta A, Mathur SK, Reddy CP, Arora B: Testicular granulosa cell tumor, adult type. Indian J Pathol Microbiol. 2008, 51: 405-406.
Hammerich KH, Hille S, Ayala GE, Wheeler TM, Engers R, Ackermann R, Mueller-Mattheis V: Malignant advanced granulosa cell tumor of the adult testis: case report and review of the literature. Hum Pathol. 2008, 39: 701-709.
Song Z, Vaughn DJ, Bing Z: Adult type granulosa cell tumor in adult testis: report of a case and review of the literature. Rare Tumors. 2011, 3: e37-
Hanson JA, Ambaye AB: Adult testicular granulosa cell tumor: a review of the literature for clinicopathologic predictors of malignancy. Arch Pathol Lab Med. 2011, 135: 143-146.
Susil BJ, Sumithran E: Sarcomatous change in granulosa cell tumor. Hum Pathol. 1987, 18: 397-399.
Kabukcuoglu F, Sungun A, Sentürk BA, Evren I, Ilhan R: Mixed germ cell tumor of the ovary with sarcomatous component. Pathol Oncol Res. 2001, 7: 60-62.
McNeilage J, Alexiadis M, Susil BJ, Mamers P, Jobling T, Laslett G, Trajstman A, Fuller PJ: Molecular characterization of sarcomatous change in a granulosa cell tumor. Int J Gynecol Cancer. 2007, 17: 398-406.
Gilcrease MZ, Delgado R, Albores-Saavedra J: Testicular Sertoli cell tumor with a heterologous sarcomatous component: immunohistochemical assessment of Sertoli cell differentiation. Arch Pathol Lab Med. 1998, 122: 907-911.
Lima JF, Jin L, de Araujo AR, Erikson-Johnson MR, Oliveira AM, Sebo TJ, Keeney GL: Medeiros F FOXL2 mutations in granulosa cell tumors occurring in males. Arch Pathol Lab Med. 2012, 136: 825-828.
Young RH, Scully RE: Testicular Tumors. 1990, Chicago: ASCP Press
Sourial MW, Sabbagh R, Doueik A, Ponsot I: A 17 year old male with a testicular fibrothecoma: a case report. Diagn Pathol. 2013, 8: 152-
Mikuz G, Colecchia M: Teratoma with somatic-type malignant components of the testis. A review and an update. Virchows Arch. 2012, 461: 27-32.
Gordon MD, Corless C, Renshaw AA, Beckstead J: CD99, keratin, and vimentin staining of sex cord-stromal tumors, normal ovary, and testis. Mod Pathol. 1998, 11: 769-773.
Yao DX, Soslow RA, Hedvat CV, Leitao M, Baergen RN: Melan-A (A103) and inhibin expression in ovarian neoplasms. Appl Immunohistochem Mol Morphol. 2003, 11: 244-249.
Völker HU, Engert S, Cramer A, Schmidt M, Kämmerer U, Müller-Hermelink HK, Gattenlöhner S: Expression of CD56 isoforms in primary and relapsed adult granulosa cell tumors of the ovary. Diagn Pathol. 2008, 3: 29-
Mosharafa AA, Foster RS, Bihrle R, Koch MO, Ulbright TM, Einhorn LH, Donohue JP: Does retroperitoneal lymph node dissection have a curative role for patients with sex cord-stromal testicular tumors?. Cancer. 2003, 98: 753-757.
The authors received no financial support for the research, authorship, and/or publication of this article.
The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
GM and TS carried out histological and immunohistochemical analysis of the tumor. GM wrote the pathological part of the manuscript and TS helped to draft the manucript. RS and AH performed molecular analysis of tumor tissue and took part in drafting the molecular part of the manuscript. ML and SS carried out clinical diagnostics and wrote the clinical part of the manuscript. All authors read and approved the final manuscript.
About this article
Cite this article
Schubert, T.E., Stoehr, R., Hartmann, A. et al. Adult type granulosa cell tumor of the testis with a heterologous sarcomatous component: case report and review of the literature. Diagn Pathol 9, 107 (2014). https://doi.org/10.1186/1746-1596-9-107
- Granulosa Cell
- Stromal Tumor
- Granulosa Cell Tumor
- Adult Type