Open Access

Severe congenital nemaline myopathy with primary pulmonary lymphangiectasia: unusual clinical presentation and review of the literature

  • Jariya Waisayarat1Email author,
  • Chinnawut Suriyonplengsaeng1, 3,
  • Chaiyos Khongkhatithum2 and
  • Mana Rochanawutanon1
Diagnostic Pathology201510:27

https://doi.org/10.1186/s13000-015-0270-8

Received: 7 November 2014

Accepted: 7 April 2015

Published: 16 April 2015

Abstract

Introduction

Nemaline myopathy is a rare genetic muscle disorder defined by the presence of nemaline rods in the muscle fibre sarcoplasm. Congenital nemaline myopathy is the most serious form of the disease’s spectrum.

Case presentation

The affected newborn has no spontaneous movement, fractures at birth and respiratory insufficiency. The present case was a Thai male, floppy at birth with fractures of both humeri and femurs and ventilator-dependent respiration. The patient developed bilateral chylothorax two weeks later and died at the age of 6 weeks. Whole-body postmortem examination with informed consent and genetic analysis of ACTA1 mutation were performed. A skeletal muscle biopsy examined by light and transmission electron microscopy showed the features of nemaline myopathy. ACTA 1 heterozygous missense mutation (c.1127G > C) was identified. Histological examination of both lungs revealed primary pulmonary lymphangiectasia.

Conclusion

To the best of our knowledge, congenital nemaline myopathy with primary pulmonary lymphangiectasia causing bilateral chylothrax has never been previously reported. Considering chylothorax as a poor prognostic index and an unusual clinical presentation of severe congenital NM are proposed.

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Keywords

Severe congenital nemaline myopathyCongenital myopathy ACTA1 ChylothoraxPrimary pulmonary lymphangiectasia

Background

The hallmark of all nemaline myopathies (NM), regardless of the genetic defect, is the presence of intrasarcoplasmic rod-like structures called nemaline bodies/rods which are stained red on the modified Gömöri trichrome preparation. This disorder was first recognized in 1963 by Shy and colleagues [1]. They proposed the term “nemaline myopathy” derived from the Greek word “nema” meaning thread. At the present time, NM is a well-recognized myopathy involving multiple genetic defects and encompassing a broad clinical spectrum varying from mild to severe muscle weakness. Affected patients are classified into six types based on the age at onset and the severity of motor and respiratory involvement according to the classification of Wallgren-Pettersson and colleagues [2,3]. Congenital nemaline myopathy is the most serious form of the disease’s spectrum. The affected newborn has no spontaneous movement, fractures at birth and respiratory insufficiency. Mutations in eight genes have now been identified in NM; ACTA1, NEB, TPM2, TPM3, TNNT1, CFL2, KBTBD13 and KLHL40 [4,5]. NM is listed as a “rare disease” by the Office of Rare Diseases of the National Institutes of Health. We present the postmortem examination of a case of congenital NM with the additional finding of bilateral chylothorax. Only two cases of severe congenital NM with chylothorax have been documented in the literature [6,7]. However, no cause of chylothorax was identified in both cases described in the literature. We discuss the possible mechanism of chylothorax in NM. Chylothorax is also a rare condition defined as the accumulation of chyle in the pleural space. Aetiologies of chylothorax can be mainly classified as traumatic or non traumatic [8]. Non traumatic causes are further subclassified as malignancy, diseases, congenital malformation or idiopathic. Moreover, congenital disorders and trauma due to intrathoracic surgery are responsible for the pathogenesis of neonatal chylothorax in most cases [9].

Case presentation

A male infant with generalized symmetrical hypotonia was born to non-consanguineous parents. The mother was a primigravida 22-year-old and the father was a healthy 28-year-old. Five older sisters and three older brothers of the maternal grandfather had died within the first year of life ranging from stillbirth to 7 months old without clarified cause of death. A cesarean section was carried out in the 37th week of gestation because of breech presentation. Closed fractures of both humeri and femurs had occurred during delivery. Plaster casts were applied. The baby had absent spontaneous movement and difficult breathing. Apgar scores of 4 and 6 were documented at the 1st and 5th minutes, respectively. Eye movements were unimpaired. Intubation and ventilatory support were required. Weight, crown-heel-length and head circumference were within normal limits (2,750 g, 47 cm and 37 cm, respectively). Neurological deficits were observed; absent primitive reflexes (Moro, Babinski and grasping reflexes), loose anal sphincter tone and areflexia of all four limbs. Two weeks after birth, the patient developed bilateral pleural effusions (Figure 1) subsequently diagnosed as bilateral chylothorax due to the high triglyceride level (4,773 mg/dl) of the pleural fluid. No clinical evidence of heart failure was documented. Serum creatine kinase was normal (16 and 25 U/L). Spinal muscular atrophy was excluded by analysis of the SMN gene in which no mutations were found. Muscle biopsy from the right quadriceps femoris was performed at the age of 1 month. Despite the symptomatic treatment with ventilator support, intercostal drainage and parenteral nutrition, the patient still suffered from respiratory insufficiency and finally died at the age of 6 weeks. Informed consent for postmortem examination was formally obtained.
Figure 1

Chest radiograph demonstrated bilateral pleural effusion at 2 weeks of age. Closed fractures of both humeri were noted.

Postmortem examination

A high-arched palate and micrognathia were observed. Plaster casts of all fracture sites were noted. Generalized mild pitting oedema was present. Internal examination of the thoracic cavity revealed minimal milky fluid in both pleural cavities and severe pleural adhesions. The lung weight (60 g in total) and anatomy were normal. The thoracic duct rupture was not apparent. The cut surfaces of both lungs showed many dilated spaces measuring up to 0.2 cm in diameter. No structural cardiac anomaly was observed. Both testes were identified at the internal openings of the inguinal canals. Brain examination showed a normal external configuration with mild non-obstructive hydrocephalus revealed on slicing. Muscle samples from the biceps brachii, intercostal muscles, diaphragm and quadriceps femoris were obtained.

Microscopic examination

All muscle tissue obtained before and after death were preserved using a snap freezing technique. Frozen sections (10 microns) were cut and stained using a panel of tinctorial and enzyme histochemical methods according to standard protocols; haematoxylin and eosin (H&E), modified Gömöri trichrome (mGt), nicotinamide adenine dinucleotide - tetrazolium reductase (NADH-TR), cytochrome oxidase (COX), succinate dehydrogenase (SDH), COX/SDH, periodic acid-Schiff (PAS), PAS with diastase (PASD), oil red O, myophosphorylase, alkaline phosphatase, adenylate deaminase and ATPase at pH 4.3, 4.6 and 9.4.

Routine histology of the muscle demonstrated well populated fascicles and marked variation in muscle fibre size (4-40 micron). There were mild increase in internal nuclei (6% of fibres), scattered atrophic and regenerating fibres, and moderate increase in endomysial and perimysial connective tissue. Split, whorled, necrotic and ragged red fibres were absent. Numerous nemaline rods were highlighted with mGt staining in almost type I fibres (Figure 2A). Transmission electron microscopy demonstrated copious sarcoplasmic electron dense nemaline rods (Figure 2B). Rods appeared either as extensions of sarcomeric Z-lines, in random array without obvious attachment to Z-lines, or in large clusters localized at the sarcolemma or intermyofibrillar spaces. The diagnosis of nemaline myopathy was established considering the clinical presentation and muscle pathology. Routine histology of the sampled tissue from each organ was performed. Microscopic examination of both lungs showed mature alveoli compatible with the alveolar stage of lung development. Diffuse dilatation of existing lymphatic vessels, without an increase in their number or complexity, were bilaterally evident in the subpleural connective tissue, interlobular septa and peribronchovascular areas (Figure 2C, D). The appearance was compatible with pulmonary lymphangiectasia rather than lymphangiomatosis. Positive immunoreactivity for CD31 and CD34 (low level) of the endothelial cells lining dilated lymphatic vessels was demonstrated. The hyaline membrane, a feature of diffuse alveolar damage, was absent. The CD31-and-CD34-immunoreactive endothelium of the dilated lymphatic vessels was also identified in the sections of pancreas. A choroid plexus papilloma 1 cm in diameter with features of a WHO grade I lesion was present in the left lateral ventricle giving rise to the documented communicating hydrocephalus.
Figure 2

Summary of investigation. (A) The quadriceps femoris biopsy showed multiple red nemaline rods in sarcoplasm. mGt, X1000. (B) Electron micrograph revealed many nemaline rods (arrow) of uneven size. X30000. (C) Dilated lymphatic vessels (star) were noted in the pleura and interlobular septum. H&E, X100. (D) Low power demonstrated markedly-dilated lymphatic vessels (star) in peribronchovascular areas. Some were more than 5 times of the adjacent alveolar diameter. H&E, X40. (E) Normal sequence of the ACTA 1 gene (control). (F) A heterozygous substitution of G to C at nucleotide position 1127 (c.1127G > C) in exon 7 of ACTA1 gene was evident.

Genetic analysis

ACTA1 gene analysis was performed on DNA derived from blood. Mutation analysis in 6 coding exons (exon 2-7) of ACTA1 gene was performed by PCR amplification, followed by direct DNA sequencing. A heterozygous substitution of G to C at nucleotide position 1127 (c.1127G > C) in exon 7 of ACTA1 was identified, resulting in a Cys to Ser substitution at codon 376 (Cys376Ser) (Figure 2E,F). No ACTA1 mutation was evident in blood samples from either parent.

Discussion

The ACTA1 gene locating on chromosome 1 (1q42.13) normally encodes skeletal muscle α-actin protein, which is an essential component of sarcomeres in skeletal muscle fibres. Actin proteins are not only important for cell movement and muscle contraction, but also help maintaining the cytoskeleton determining cell shape and organizing cellular contents. Mutation of ACTA1 results in various types of congenital myopathy. ACTA1 heterozygous missense mutation combined with the microscopic finding of nemaline rods in the sarcoplasm warrants the diagnosis of NM in the present case. This genetic defect represents a dominant mutation arising de novo as neither parent carried the mutation. This pattern of inheritance is commonly identified in NM cases caused by the mutation in ACTA1 [4,10,11].

Due to the absence of spontaneous neonatal movement, respiratory insufficiency and fractures of both humeri and femurs at birth, NM in this patient was categorized as the severe congenital type [2,3]. The disease caused additional neurological deficits (absent primitive reflexes, loose anal sphincter tone and areflexia of all extremities). The affected diaphragm, confirmed by the presence of numerous nemaline rods, resulted in the ventilator-dependency from birth. This respiratory insufficiency was well correlated with the study data of Ryan MM and colleagues [3] in that all 23 reviewed cases with severe congenital NM had significant respiratory problems and twelve were ventilator-dependent.

Pulmonary pathology consisted of diffuse bilateral pulmonary lymphangiectasia. Absent clinical signs and symptoms of heart failure and passive congestion of liver and spleen supported the diagnosis of primary or congenital pulmonary lymphangiectasia (PPL). PPL is a rare congenital disease of unknown cause characterized by abnormal pulmonary lymphatic dilatation in the absence of secondary lymphatic obstruction [12]. Bilateral chylothorax had been clinically observed in the present case. Chylothorax is defined as the accumulation of lymphatic fluid in the pleural space. In neonatal chylothorax both congenital and traumatic (especially after thoracic surgery) forms have been recognized. Congenital chylothorax in some cases is associated with Turner syndrome, Noonan syndrome, Down syndrome, congenital lymphangiectasia or hydrops fetalis [9]. Despite no obvious evidence of microscopic pleural rupture in this case, the most likely cause of the bilateral chylothorax was PPL. The association between PPL and congenital chylothorax had been well documented [13,14]. Neonatal onset PPL without additional anomalies is now compatible with life and has a good prognosis in the ventilator era of neonatal intensive care [12]. In this case PPL with chylothorax may have been aggravated by the hypotonic diaphragm due to NM. Prolonged intubation since birth causing compression to the thoracic duct leading to increased hydrostatic pressure in the thoracic duct and subsequent chylous leakage is another possible additional cause of chylothorax in the present case.

Our case represents the third example of severe congenital NM with chylothorax to be documented in the literature [6,7], however, the etiology of chylothorax in those two cases has yet to be established. To the best of our knowledge, severe congenital NM with PPL causing bilateral chylothrax has never been previously reported. Two further cases of congenital myopathy (myotubular myopathy, congenital myotonic dystrophy type 1) with chylothorax of unknown cause have also been reported [15,16]. The reported cases are summarized in Table 1. All 3 reported cases of NM with chylothorax were male with severe congenital presentation of myopathy and de novo dominant mutation in ACTA1. Among these three cases of NM with chylothorax, none survived more than 9 weeks indicating a poor prognosis in this group and suggesting that this represents a poor prognostic sign. Moreover, recognition that chylothorax can be an unusual clinical manifestation of severe congenital NM, or other myopathies, indicates that myopathy should be included in the differential diagnosis of chylothorax in the neonate.
Table 1

Clinical data of case reports of congenital myopathy with chylothorax

Study

Authors’ study

Garcia-Angarita, et al. (2009) [ 7 ]

Schröder, et al. (2004) [ 6 ]

Smets (2008) [ 15 ]

Son, et al. (2012) [ 16 ]

Age at presentation

At birth (GA 37 week)

At birth (GA 39 week)

At birth

At birth

At birth, (GA 30 week, non-identical twin A)

Sex

Male

Male

Male

Male

Male

Race

Thai

German

Turkish

Turkish

Korean

Diagnosis

Nemaline myopathy, severe congenital

Nemaline myopathy, severe congenital

Nemaline myopathy (intranuclear rod variant), severe congenital

Myotubular myopathy, severe congenital

Congenital myotonic dystrophy type 1

Gene muatation

ACTA1 gene (c.1127G > C, exon 7), heterozygous

ACTA1 gene (c.222G > T & c.223C > T, exon 3), heterozygous

ACTA1 gene (Asp154Asn, exon 4), heterozygous

MTM1 gene (c.1261-10A > G)

DMPK gene (more than 750 CTG repeats)

Inheritance

De novo

De novo

De novo

X-linked recessive

Autosomal dominant (affected mother)

Chylothorax

Bilateral

Bilateral

Present

Bilateral

Bilateral

Triglyceride level in pleural effusion (mg/dl)

4773

NA

NA

746

495

Cause of chylothorax

Primary pulmonary lymphangiectasia

Not defined

Not defined

Not defined

Not defined

Serum creatine kinase (U/L)

16-25, Low, (normal 30-200 U/L)

Normal

NA

NA

281

Signs and symptoms

Absent spontaneous neonatal movement, respiratory difficulty, fractures of both humeri and femurs, high arch palate

Absent spontaneous neonatal movement, respiratory difficulty, fractures of both humeri and femurs

Generalized hypotonia, respiratory difficulty, right pulmonary hypoplasia

Generalized hypotonia, respiratory difficulty

Generalized hypotonia, respiratory difficulty, triangular face, inverted v-shaped upper lip

Age at death, Cause of death

6 weeks, respiratory failure

8 weeks, respiratory failure

9 weeks, respiratory failure

16 weeks, NA

14 weeks, respiratory failure and pneumonia

GA, gestational age; NA, not available.

Conclusion

We report a case of congenital nemaline myopathy presenting with classical features of the disease (fractures, respiratory insufficiency and absence of spontaneous movement at birth) combined with unusual presentation (bilateral chylothorax due to primary pulmonary lymphangiectasia). The presence of nemaline rods in the skeletal muscle biopsy examined by light and electron microscopy, and ACTA1 mutation analysis (c.1127G > C) confirmed this muscular disorder. Lung histological examination revealed diffuse dilatation of existing lymphatic vessels. Together with the absence of secondary causes, this lung pathology was diagnosed as primary pulmonary lymphangiectasia. Recognition that chylothorax can be an unusual clinical manifestation of severe congenital NM, or other myopathies, indicates that myopathy should be included in the differential diagnosis of chylothorax in the neonate.

Consent

Written informed consent was obtained from the patient’s parent for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.

Abbreviations

ACTA1

Alpha-skeletal actin gene

COX: 

Cytochrome oxidase

H&E: 

Haematoxylin and eosin

mGt: 

Modified Gömöri trichrome

NADH-TR: 

Nicotinamide adenine dinucleotide-tetrazolium reductase

NM: 

Nemaline myopathy

PAS: 

Periodic acid-Schiff

PASD: 

PAS with diastase

PPL: 

Primary or congenital pulmonary lymphangiectasia

SDH: 

Succinate dehydrogenase

Declarations

Acknowledgements

We wish to thank the patient’s parents who donated their beloved son for medical academic purpose. We are grateful to Prof. Janice Holton (UCL Institute of Neurology, The National Hospital For Neurology and Neurosurgery, England) for editing the manuscript, and to Dr. Chanin Limwongse (Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand) for genetic analysis. The technical assistance of Nutta Thubthong, Pavinee Rittilarp and Suda Sanpapant are greatly acknowledged.

Authors’ Affiliations

(1)
Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University
(2)
Department of Pedriatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University
(3)
Department of Anatomy, Faculty of Science, Mahidol University

References

  1. Shy GM, Engel WK, Somers JE, Wanko T. Nemaline myopathy. A new congenital myopathy. Brain. 1963;86:793–810.View ArticlePubMedGoogle Scholar
  2. Wallgren-Pettersson C, Laing NG. Report of the 70th ENMC International Workshop: nemaline myopathy. Neuromuscul Disord. 2000;10:299–306.View ArticlePubMedGoogle Scholar
  3. Ryan MM, Schnell C, Strickland CD, Shield LK, Morgan G, Iannaccone ST, et al. Nemaline Myopathy - A Clinical Study of 143 cases. Ann Neurol. 2001;50(3):312–20.View ArticlePubMedGoogle Scholar
  4. Wallgren-Pettersson C, Sewry CA, Nowak KJ, Laing NG. Nemaline myopathies. Semin Pediatr Neurol. 2011;18(4):230–8.View ArticlePubMedGoogle Scholar
  5. Ravenscroft G, Miyatake S, Lehtokari VL, Todd EJ, Vornanen P, Yau KS, et al. Mutations in KLHL40 are a frequent cause of severe autosomal-recessive nemaline myopathy. Am J Hum Genet. 2013;93(1):6–18.View ArticlePubMed CentralPubMedGoogle Scholar
  6. Schröder JM, Durling H, Laing N. Actin myopathy with nemaline bodies, intranuclear rods, and a heterozygous mutation in ACTA1 (Asp154Asn). Acta Neuropathol. 2004;108(3):250–6.View ArticlePubMedGoogle Scholar
  7. Garcia-Angarita N, Kirschner J, Heiliger M, Thirion C, Walter MC, Schnittfeld-Acarlioglu S, et al. Severe nemaline myopathy associated with consecutive mutations E74D and H75Y on a single ACTA1 allele. Neuromuscul Disord. 2009;19(7):481–4.View ArticlePubMedGoogle Scholar
  8. McGrath EE, Blades Z, Anderson PB. Chylothorax: Aetiology, diagnosis and therapeutic options. Respir Med. 2010;104:1–8.View ArticlePubMedGoogle Scholar
  9. Van Straaten HL, Geralds LJ, Krediet TG. Chylothorax in the neonatal period. Eur J Pediatr. 1993;152(1):2–5.View ArticlePubMedGoogle Scholar
  10. Agrawal PB, Strickland CD, Midgett C, Morales A, Newburger DE, Poulos MA, et al. Heterogeneity of nemaline myopathy cases with skeletal muscle alpha-actin gene mutations. Ann Neurol. 2004;56(1):86–96.View ArticlePubMedGoogle Scholar
  11. Wallgren-Pettersson C, Pelin K, Nowak KJ, Muntoni F, Romero NB, Goebel HH, et al. Genotype-phenotype correlations in nemaline myopathy caused by mutations in the genes for nebulin and skeletal muscle α-actin. Neuromuscul Disord. 2004;14(8-9):461–70.View ArticlePubMedGoogle Scholar
  12. Barker PM, Esther Jr CR, Fordham LA, Maygarden SJ, Funkhouser WK. Primary pulmonary lymphangiectasia in infancy and childhood. Eur Respir J. 2004;24(3):413–9.View ArticlePubMedGoogle Scholar
  13. Lee SY, Yang SR, Lee KR. Congenital pulmonary lymphangiectasia with chylothorax. Asian Cardiovasc Thorac Ann. 2002;10(1):76–7.View ArticlePubMedGoogle Scholar
  14. Moerman P, Vandenberghe K, Devlieger H, Van Hole C, Fryns JP, Lauweryns JM. Congenital pulmonary lymphangiectasis with chylothorax: a heterogeneous lymphatic vessel abnormality. Am J Med Genet. 1993;47(1):54–8.View ArticlePubMedGoogle Scholar
  15. Smets K. X-linked myotubular myopathy and chylothorax. Neuromuscul Disord. 2008;18(2):183–4.View ArticlePubMedGoogle Scholar
  16. Son SB, Chun JM, Kim KA, Ko SY, Lee YK, Shin SM. A case report on 30-week premature twin babies with congenital myotonic dystrophy conceived by in vitro fertilization. J Korean Med Sci. 2012;27(10):1269–72.View ArticlePubMed CentralPubMedGoogle Scholar

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© Waisayarat et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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