- Open Access
EGFR T790M detection and osimertinib treatment response evaluation by liquid biopsy in lung adenocarcinoma patients with acquired resistance to first generation EGFR tyrosine kinase inhibitors
© The Author(s). 2018
- Received: 21 May 2018
- Accepted: 30 July 2018
- Published: 13 August 2018
Lung adenocarcinoma with EGFR activating mutations will inevitably acquire resistance to first generation TKIs. Acquired EGFR T790M mutation causes about 50% of these resistance cases. Droplet digital PCR (ddPCR) and cobas enables quantification of T790M mutation. Whether these methods can predict clinical response of osimertinib treatment is unknown.
Tumor and blood samples from 69 stage IIIB-IV NSCLC patients acquired resistance to EGFR-TKI were collected. Cobas® Mutation Test v2 kit was used to detect EGFR mutations in FFPE or plasma samples. Plasma T790M mutation of both osimertinib naïve and treated patients were quantified by Droplet digital PCR (ddPCR).
T790M mutation rate detected by FFPE tissue cobas, plasma ctDNA cobas and plasma ctDNA ddPCR test were 54.5, 21.3 and 30.4% respectively. The T790M positive rate was 52.2% considering all testing methods. The objective response rate (ORR) was 60.9% in 23 patients received osimertinib treatment. Quantification of T790M after treatment decreased to very low level, but no association was observed between clinical response and T790M mutation level decrease.
ddPCR is more sensitive in plama ctDNA testing and should be performed even in tumor tissue T790M test negative cases. EGFR T790M mutation level is not associated with clinical response after osimertinib treatment.
- Epidermal growth factor receptor
Part result of this study was published as abstract and poster presentation in IASLC 18th World Conference on Lung Cancer .
Lung cancer remains the leading cause of cancer-related death worldwide . Though epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) have significantly improved outcome of EGFR mutation positive advanced non-small cell lung cancer (NSCLC), most of these patients develop resistance to first generation EGFR TKIs after a median of 10–12 months, in which about 50–60% of these tumors harbor EGFR T790M resistance mutation [3–5]. Osimertinib is an oral, irreversible EGFR-TKI that is selective for both EGFR activating and T790M resistance mutations . In AURA3 study, osimertinib showed significant advantage against platinum therapy plus pemetrexed in both progression-free survival and objective response rate .
To obtain enough tumor tissue in patients experienced disease progression is challenging, especially in patients receiving first generation EGFR-TKI treatment. In other cases, tumor biopsy tissue may not reflect the overall tumor mutational features due to high tumor burden and heterogeneity. The use of circulating tumor DNA (ctDNA) to detect genetic alterations by various platforms known as liquid biopsy has provided a non-invasive option . The current study focuses on the efficacy of T790M detection by different platforms, as well as T790M level response after receiving osimertinib treatment.
Plasma and tumor tissue samples were collected from patients screened or enrolled in the ongoing ASTRIS study. ASTRIS (D5160C00022) is open label, multinational, multicenter, real world treatment study of single agent osimertinib for patients with advanced/metastatic EGFR T790M mutation positive NSCLC who have received prior therapy with an first generation EGFR-TKI. Patients were required to have the diagnosis of EGFR activating mutation positive NSCLC. Drug resistance was defined as disease progression after first generation EGFR-TKI (gefetinib, elortinib or ecotinib) treatment, which was evaluated by RECIST 1.1 criteria. EGFR T790M positive patients received osimertinib 80 mg Qd until disease progression evaluated by investigators.
Tumor tissue and plasma sample collection and mutational analysis
Tumor tissue or plasma samples were collected from patients screened or enrolled in ASTRIS study, following progression on a previous EGFR-TKI, but prior to dosing with osimertinib. CT-guided needle lung biopsy or Supraclavicular lymph node biopsy samples were snap frozen and stored at − 80 °C. A total of 8 ml of Plasma sample from each patient were assigned for cobas and ddPCR testing, and 4 ml of plasma from the time point of first evaluation (6 weeks) after osimertinib treatment were evaluated by ddPCR. ctDNA isolation followed standard protocols. Cobas® Mutation Test v2 kit was used to detect both EGFR activating mutation and T790M in FFPE or plasma samples. The Cobas is an allele-specific polymerase chain reaction assay to detect known common EGFR mutations. Analysis was confirmed by negative and positive controls. The PCR reactions were run on the cobas®z 480 analyzer with EGFR Blood Analysis Package Software. A semiquantitative index (SQI) was generated to reflect the proportion of mutated and wild-type copies of the EGFR gene. The SQI was derived from a dilution series with known copy numbers of mutated EGFR and a fixed amount of wild-type EGFR, with the wildtype DNA copy as internal control. Bio-rad PrimePCR™ ddPCR™ Mutation Assay kit were used to detect T790M in plsma ctDNA. The RNase P Copy Number Reference Assay (Life Technologies, Carlsbad, California) was used to determine the total amount of DNA in plasma samples. A total of 3000–3500 genome equivalents were analyzed per reaction with a sensitivity between 0.1–0.5%.
Associations between mutations and clinical and biological characteristics were analyzed by χ2 or Fisher’s exact test. All data were analyzed using the Statistical Package for the Social Sciences Version 16.0 Software (SPSS Inc., Chicago, IL). The two-sided significance level was set at p < 0.05.
Study design and patient characteristics
Demographics and clinicopathologic features of 69 NSCLC patients screened for T790 M mutation
No. of patients (%)
ECOG performance status
First generation EGFR TKIs
Two or more TKI drugs
EGFR mutation detection
FFPE slieds by cobas
cfDNA by cobas
T790 M ddPCR assay
Detection of T790M mutation by cobas® and ddPCR platform
Association between EGFR T790 M mutation and clinical features in patients with disease progression after receiving 1st generation EGFR TKI treatment
EGFR T790M detected by any method
EGFR T790M detected by plama ctDNA ddPCR
T790M detected by cobas plama ctDNA test
Cobas Activating Mutation SQI
Cobas T790M SQI
ddPCR T790M quantitative
Time of EGFR-TKI treatment
≤ 6 months
Evaluation of plasma T790M level during osimertinib treatment by ddPCR
The aim of this study was to evaluate different T790M detecting methods in advanced NSCLC patients who experienced disease progression after receiving EGFR TKI treatment, as well as T790M quantification after osimertinib treatment. Two quantification methods were tested on a cohort of 69 patients enrolled in this single center as part of the multicenter real-world ASTRIS study. These patients represent outline features of Chinese patients who experienced disease progression after gefetinib, elortinib or ecotinib treatment. Plasma samples were collected at screening and 6 weeks after receiving osimertinib treatment. The overall T790M positive rate was 52.2% considering all testing methods, the ORR of T790M positive patients receiving osimertinib treatment was 60.9%. These data were similar compared with published data [7, 9–11].
Our analysis revealed a rising trend of T790M positive rates detected by ddPCR in stage IIIB, IVA and IVB patients. In plasma ctDNA samples tested by cobas, T790M positive rate was significantly higher in stage IVB than stage IIIB and IVA, M1c than M1a and M1b patients. On one side, more advanced stage represents significantly higher tumor burden, in which case tumor shed more ctDNA to the bloodstream [12, 13]. On the other side, these results indicate that the cobas test is less capable of detecting relatively earlier stage cases.
In all of the plasma ctDNA cobas test T790M positive samples, ddPCR test also yielded positive results. Even in 10 tumor tissue test negative cases, 3 were positive defined by plasma ctDNA ddPCR test. These results suggest that plasma ctDNA ddPCR test is more sensitive and should be used as primary choice in managing patients with resistance to first line EGFR TKIs. The reason of inconsistency between tumor tissue test and ddPCR test is probably due to tumor heterogeneity in primary and metastatic tumors, as well as intratumor heterogeneity. These facts suggests co-existing of multiple resistant clones or single clone harboring multiple resistance mechanism [14, 15]. Plasma ctDNA ddPCR test should be routinely performed in such cases considering its noninvasive and low cost feature.
Most of patients showed a PR or SD status after the evaluation of 6 weeks after receiving osimertinib treatment, generating an ORR of 60.9%. We also compared the ctDNA T790M level in pre and post osimertinib treatment plasma samples. Though all plasma ctDNA T790M decreased to very low level, no association was observed with radiographic response. Previous studies dynamically monitored EGFR mutation status using plasma samples by ddPCR to evaluate response to first generation EGFR TKIs . Another study quantified plasma T790M level in two cases of patients who received osimertinib treatment . In the present study, we have quantified T790M level in relatively large number of samples. Though quantification of plasma ctDNA T790M didn’t predict response in short term, dynamic monitoring may indicate disease progression in the long run.
In conclusion, our data suggest that ddPCR is more sensitive in plama ctDNA testing and should be performed even in tumor tissue T790M test negative cases. Osimertinib significantly decreased plasma T790M level, but no association was observed between plasma ctDNA T790M level decrease and clinical response.
We thank Yongsheng Sha, Hao He, Lu Ye, Mei Liu, Aiping Xu and Yan Yan for managing clinical samples.
This work was supported by the National Natural Science Foundation of China (81302001, 81772484), National Key Research and Development Program of China (2016YFC0905501, 2016YFC0905500), China Postdoctoral Science Foundation (2014 M550147, 2015 T80225), Cancer translational medicine seed fund of Tianjin medical university cancer institute and hospital (Fund No.1606).
Availability of data and materials
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
CG L, RJ, BZ and HL L analyzed patient and experiment data. RJ and CG L was the major contributor in writing the manuscript. CL W and CG L designed and supervised the research. All authors read and approved the final manuscript.
Ethics approval and consent to participate
This study was conducted in accordance with the Declaration of Helsinki. All patients provided written informed consent before screening. The ASTRIS study protocol was reviewed and approved by review boards of Tianjin medical university cancer institute and hospital.
Consent for publication
The authors declare that they have no competing interests.
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