Fig. 3

Sequencing and identification of mutations in the viral S gene. Comparisons of the reference sequence of the S gene and the sequence obtained from the patient’s oropharyngeal and nasopharyngeal swab are shown, at the sites where the two mutations were found. A The change of an adenine for a guanine at position 1841 (A1841G) is highlighted in yellow, and the electropherogram obtained where this mutation was found (black arrow) is shown. B The change of a cytosine for a thymine in position 3172 (C3172T) and the electropherogram where this mutation was found. C Three-dimensional structural visualization of the viral S protein with the amino acid changes identified, shown as colored balls: mutation D614G in yellow and mutation H1058Y in blue. Lateral view (left), upper (center) and lateral view bound to the ACE-2 receptor (in green, right)