- Open Access
Combined effects of 17-DMAG and TNF on cells through a mechanism related to the NF-kappaB pathway
© Qu et al.; licensee BioMed Central Ltd. 2013
Received: 7 January 2013
Accepted: 22 April 2013
Published: 1 May 2013
The tumor necrosis factor (TNF) and the cellular NF-κB pathway protein IKKβ play important roles in various cellular processes such as cell proliferation, survival, differentiation, and apoptosis. A heat shock protein 90 inhibitor, 17-DMAG, can induce apoptosis of some tumor cells. This study is to determine the combined effects of 17-DMAG and TNF on malignant cells and the related mechanisms.
We have determined effects of 17-DMAG, an Hsp90 inhibitor, and TNF treatments on the small cell lung cancer cell line (MS-1), the adenocarcinoma cell line (A549), the squamous-cell carcinoma cell line (LK-2), and the normal human bronchial epithelium cell line (NuLi-1) by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrozolium bromide assay. To determine if 17-DMAG inhibit the expression of IKKβ in the normal human NuLi-1 cells, and the malignant MS-1, A549, and LK-2 cells, immunoblotting assays and luciferase assays were performed.
It was found that the combined treatments resulted in synergistic killing of malignant cells, which was confirmed by the apoptosis determination using a fluorescence microscopic assay following staining of the drug-treated cells with Hoescht 33258. The immunoblotting results indicated that the synergistic killing due to 17-DMAG and TNF treatments may be related to the decreases in IKKβ levels in the presence of 17-DMAG.
The results suggest that combination of 17-DMAG and TNF treatments might be useful for treating malignancies upon further study in the further.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2041198513886824
Heat shock proteins (Hsps) are a group of chaperones that are important in maintaining stability and function of their client proteins. Heat shock protein 90 (Hsp90) regulates stability, degradation, translation, and function of its client proteins, via an ATP-regulated mechanism . Unlike other Hsps, only approximately 200 cellular proteins are determined to be clients of Hsp90 [2–4]. Hsp90 inhibitors, such as 17-DMAG, are a group of small molecules that bind to the ATP-binding pocket of the Hsp90 dimers [5, 6]. By competing with ATP, 17-DMAG can inhibit the protein chaperoning activity of Hsp90, resulting in misfolding of cellular client proteins such as the tyrosine kinase v-Src, the serine/threonine kinase Raf, mutant p53 proteins, the glucocorticoid receptor, and the androgen receptor HER2, resulting in protein destabilization and degradation, usually mediated by the proteasomal pathway . A number of Hsp90 clients are involved in tumor developments . In addition, the binding affinity of Hsp90 inhibitors to the ATP-binding pocket of Hsp90 are 10-flod higher in tumor cells than in normal cells . Therefore, Hsp90 inhibitors are promising therapeutic reagent for treating malignant cells.
The tumor necrosis factor (TNF) plays important roles in various cellular processes such as cell proliferation, survival, differentiation, and apoptosis . Since TNF can induce apoptosis of cells, it may be a potential therapeutic agent to treat cells. However, TNF induces multiple pathways including cell proliferation and survival processes that may facilitate tumor development . Therefore, combination of TNF and another agent will be a promising method of treating malignant cells, if this agent can down-regulate the cell proliferation property of TNF. Therefore, novel reagents are necessary to inhibit TNF-induced survival signals, possibly by resulting in a synergistic effect on malignant cells.
When TNF binds to the TNF receptors, a group of signaling pathways were activated. For example, the caspase cascade can be activated through the Fas-associated death domain protein, which leads to apoptosis . However, activation of the NF-κB pathway through the receptor-interaction protein upon binding of TNF facilitates cell survival and proliferation [11–13], which will decrease the anti-tumor apoptotic properties of TNF. Therefore, the balance of TNF-induced survival and death signaling is essential for the fates of cells in the presence of TNF.
During TNF-induced signaling, IκB kinases (IKKs), including IKKα, IKKβ, and IKKγ, are recruited to the signaling complex. The activated IKKβ then phosphorylates the NF-κB-bound IκBs, which retain NF-κB in the cytoplasm, at their regulatory region to trigger their degradation. This process releases NF-κB to allow its translocation into the nucleus and activation of its target genes [14, 15], with many of them possessing anti-apoptotic properties [16–18]. Since IKKβ has been identified as an Hsp90 client protein [19, 20], we hypothesize that combined treatments with 17-DMAG and TNF may lead to a promising anti-tumor effects if 17-DMAG can decrease the survival signaling properties of TNF.
Since TNF and 17-DMAG may be useful for treating some types of malignancies. In this study, we have determined the effects of 17-DMAG and TNF treatments on multiple cell lines. It is found that the combined treatments result in synergistic killing of cells. It is also indicated that such a synergistic killing may be related to the decreases in IKKβ levels in the presence of 17-DMAG. Our results suggest that combination of 17-DMAG and TNF might be useful for treating malignancies upon further study in the further.
Materials and methods
Cell lines and reagents
The small cell lung cancer (SCLC) cell line (MS-1), the adenocarcinoma cell line (A549), the squamous-cell carcinoma cell line (LK-2), and the normal human bronchial epithelium cell line (NuLi-1) were provided by Shanghai Cell Biology Institute (China). These lung cancer cell lines were maintained in RPMI-1640 medium (Sigma-Aldrich Co. Ltd, Irvine, CA) supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin at 37°C with 5% CO2 and 100% humidity. 17-DMAG (InvivoGen, San Diego, CA) and TNF-α (TNF) were dissolved in DMSO. Recombinant Human TNF was purchased from Promega.
Cell treatments and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrozolium bromide (MTT) assay
Briefly, cells at a density of 1 × 105 cells/well were seeded into 6-well plates in RPMI-1640 supplemented with 10% calf serum and were cultured for 24 h. The cells were then treated with vehicle control (DMSO, 0.016%, v/v), TNF-α (10 ng/ml), 17-DMAG (0.05 μM), or TNF-α (10 ng/ml) plus 17-DMAG (0.05 μM). At the end of each experiment, cells were incubated with 0.5 mg/ml MTT for 4 h according to the protocol of manufacturer. Viability of treated cells was expressed relative to control cells (relative viability).
Cells at a density of 1 × 105 cells/well were cultured in six-well plates in RPMI-1640 supplemented with 10% calf serum for 24 h, followed by addition of DMSO (0.016%, v/v), TNF (10 ng/ml), 17-DMAG (0.05 μM), or TNF (10 ng/ml) plus 17-DMAG (0.05 μM). After 48 h, cells were pelleted by centrifugation, washed once with PBS, fixed by incubation in 4% paraformaldehyde for 30 min at room temperature, and then washed again with PBS to remove the fixative. The fixed cells were resuspended in PBS that contained Hoescht 33258 (5 μg/ml), followed by an incubation at room temperature for 15 min in the dark. Aliquots of cells were placed on glass slides and examined for cells with apoptotic morphology (nuclear condensation and chromatin fragmentation) via fluorescence microscopy. To quantify the apoptosis, 300 nuclei from random microscopic fields were analyzed. Data are presented as the mean percentages of apoptotic cells.
Cells at a density of 1 × 105 cells/well were cultured in six-well plates in RPMI-1640 supplemented with 10% calf serum for 24 h, followed by addition of DMSO (0.016%, v/v), TNF (10 ng/ml), 17-DMAG (0.05 μM), or TNF (10 ng/ml) plus 17-DMAG (0.05 μM). After 48 h, cells were pelleted by centrifugation, washed twice with PBS. Total proteins were harvested from cells, separated on 10% SDS/PAGE gels, and then subjected to immunoblot analyses. The primary antibodies against IKKβ (about 90 kDa) and β-actin were purchased from Santa Cruz, USA (anti- IKKβ, cat# sc-8014, 1:200; anti-β-actin, cat# sc-130301, 1:10,000). Secondary antibodies used in this study were goat anti-mouse IgG-HRP (Cat# sc-2005, 1:10,000, Santa Cruz, USA). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The immunoblot experiments were repeated at least 3 times. The mean normalized optical density (OD) of HDAC protein bands relative to the OD of β-actin band from the same individual was calculated.
NuLi-1 cells, MS-1 cells, A549 cells, and LK-2 cells were plated onto 24-well plates and incubated for 20 h at 37°C in 5% CO2. The control pGL3 vector DNA (0.2 μg) (purchased from Clontech Co.) or similar amounts of Luciferase-NF-κB reporter vectors (Clontech Co., USA), was cotransfected into cells with the pRL-SV40 vector using the effectene transfection reagent (Qiagen, USA) for 20 h. Cells were then treated with DMSO (0.016%, v/v), TNF (10 ng/ml), 17-DMAG (0.05 μM), or TNF (10 ng/ml) plus 17-DMAG (0.05 μM) for 24 h. Luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega, USA) according to the manufacturer's protocol. The transcriptional activity of NF-κB in the cells transfected with the Luciferase-NF-κB reporter vectors were calculated as folds of those in the relative cells transfected with the empty vector.
The experimental data are expressed as mean ± SD. Statistical software (SPSS10.0) was used for independent sample t tests, followed by one-way variance analysis. In all analyses, P < 0.05 was considered statistically significant.
TNF-α enhances the toxic effects on tumor cells of 17-DMAG
A normal human bronchial epithelium cell line (NuLi-1) and three lung cancer cell lines (MS-1, A549, and LK-2) were treated with TNF, 17-DMAG, or both of them together for 24 hour or 48 hours. The treatment with DMSO served as a drug vehicle control. The cells were analyzed for differences in cell killing upon various treatments via number counting of living cells in the presence or absence of the above compounds.
TNF-α enhances the apoptosis induced by 17-DMAG
Since TNF-α enhances the toxic effects on tumor cells of 17-DMAG, it was determined that the effects of the drugs on apoptosis in all of these 4 types of cells. The cells were treated with either vehicle control (DMSO), TNF-α (10 ng/ml), 17-DMAG (0.05 μM), or both of TNF-α (10 ng/ml) and 17-DMAG (0.05 μM). To quantify the apoptotic incidence, we used a fluorescence microscopic assay following staining of the drug-treated cells with Hoescht 33258.
17-DMAG treatments lead to degradation of IKKβ
IKKβ expression is reported to be reduced by Hsp90 inhibitors in some cell types. To determine if 17-DMAG inhibit the expression of IKKβ in NuLi-1, MS-1, A549, and LK-2 cells, the cells were treated with either vehicle control (DMSO), TNF-α (10 ng/ml), 17-DMAG (0.05 μM), or both of TNF-α (10 ng/ml) and 17-DMAG (0.05 μM). The total proteins were extracted and the expression levels of IKKβ were determined by using immunoblot analysis, with the cellular β-actin protein serving as a loading control. The mean normalized OD of IKKβ protein bands relative to the OD of β-actin band from the same condition was all calculated and subjected to statistical analyses.
17-DMAG down-regulates the NF-κB transcriptional activity induced by TNF treatment
Next, we performed a luciferase reporter gene assay to determine whether TNF-α induces transcriptional activity of NF-κB and, if so, whether 17-DMAG affects the induction of NF-κB transcriptional activity by TNF-α. NuLi-1 cells, MS-1 cells, A549 cells, and LK-2 cells were transfected with a control pGL3 vector DNA or similar amounts of Luciferase-NF-κB reporter vectors together with the pRL-SV40 vector for 20 h.
Since many Hsp90 clients are important for disease development, 17-DMAG is extensively studied for possible treatments of various diseases [21–25]. In this study, we have determined the effects of 17-DMAG and TNF treatments on multiple cell lines. It is found that the combined treatments result in synergistic killing of malignant cells. It is also indicated that such a synergistic killing may be related to the decreases in IKKβ levels in the presence of 17-DMAG. Our results suggest that combination of 17-DMAG and TNF might be useful for treating malignancies upon further study in the further.
Combined treatments with TNF-α and 17-DMAG had not significantly decreased the viability of the normal NuLi-1 cells, but leads to obvious toxicity on malignant MS-1, A549, and LK-2 cells. This result may be related to the properties of Hsp90. As one of the most abundant proteins in the cytoplasm, Hsp90 constitutes approximately 1-2% of the total proteins , although some Hsp90 may translocate to the nucleus in response to stress and other environmental stimuli [27–30]. Under normal conditions of the normal cells, there is an abundant Hsp90 chaperone reservoir, which can buffer proteostasis against environmental stress. However, under extreme environmental conditions, such as conditions in malignant cells, the chaperone reservoir is rapidly exhausted. Therefore, effects of 17-DMAG may be increased correspondingly. In the other words, function of Hsp90 inhibitors can then affect the relationship between genotype and phenotype, and thus influencing human health, disease and evolutionary processes. This reason also explains why 17-DMAG did not affect IKKβ expression in normal cell.
Hsp90 is composed of a highly conserved amino-terminal domain, a middle domain, followed by a carboxy-terminal domain . Hsp90 binds ATP in its amino-terminal domain, hydrolyzing it upon interaction with clients and co-chaperoning molecules. Structurally unrelated inhibitors of Hsp90, such as 17-DMAG, replace ATP and specifically destroy Hsp90 function . The strong conservation of the ATP-binding pocket allows 17-DMAG to serve as extremely useful and specific tools for affecting HSP90 function. This may explain why TNF did not enhance the effect of 17-DMAG on IKKβ expression, while they exert a combined effect on viability of malignant MS-1, A549, and LK-2 cells, possibly by playing roles in different cellular signaling pathways or processes.
When cells are treated with TNF, NF-κB is activated through RIP-mediated activation of IκB kinase (IKK) and then released, allowing for its translocation into the nucleus and activation of its target genes . Many cellular targets of NF-κB, such as Bcl-xL, cIAP-1, A20, cIAP-2, and XIAP, have anti-apoptotic functions [16–18]. Therefore, many kinds of tumor cells are resistant or tolerant to TNF-induced apoptosis, although TNF may lead to apoptosis via the FADD-caspase pathway and the TRAF2-JNKK2 pathway. The NF-κB activity is considered as a major factor of TNF resistance in cancer cells . Our results clearly show that inhibition of the NF-κB pathway by 17-DMAG significantly sensitizes the TNF-treated cells to induced cell death, supporting the combined effects of 17-DMAG and TNF on cells through a mechanism related to the NF-κB pathway. In addition to the small-molecule compounds such as 17-DMAG, the siRNA strategies are often used to treat tumor cells. It is found that targeting the fascin pathway by using a specific siRNA might be a novel therapeutic strategy for curing the esophageal squamous cell carcinoma . Compounds can be also used for study of gene expression. It has been reported that gene expression profile test is useful to resolve the lung squamous cancers and other cancers such as the head and neck squamous carcinoma .
This work was supported by the following grants: Shandong province tackle key problems in science and technology program (grant NO. Y2006C14) and Qingdao municipal science and technology commission (grant No. 05-1-NS-74).
- Prodromou C, Roe SM, O'Brien R, Ladbury JE, Piper PW, Pearl LH: Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone. Cell. 1997, 90: 65-75. 10.1016/S0092-8674(00)80314-1.View ArticlePubMedGoogle Scholar
- da Silva VC, Ramos CH: The network interaction of the human cytosolic 90 kDa heat shock protein Hsp90: A target for cancer therapeutics. J Proteomics. 2012, 75: 2790-2802. 10.1016/j.jprot.2011.12.028.View ArticlePubMedGoogle Scholar
- Taipale M, Jarosz DF, Lindquist S: HSP90 at the hub of protein homeostasis: emerging mechanistic insights. Nat Rev Mol Cell Biol. 2010, 11: 515-528. 10.1038/nrm2918.View ArticlePubMedGoogle Scholar
- Theodoraki MA, Caplan AJ: Quality control and fate determination of Hsp90 client proteins. Biochim Biophys Acta. 1823, 2012: 683-688.Google Scholar
- Holzbeierlein JM, Windsperger A, Vielhauer G: Hsp90: a drug target?. Curr Oncol Rep. 2010, 12: 95-101. 10.1007/s11912-010-0086-3.View ArticlePubMedGoogle Scholar
- Pacey S, Banerji U, Judson I, Workman P: Hsp90 inhibitors in the clinic. Handb Exp Pharmacol. 2006, 172: 331-358. 10.1007/3-540-29717-0_14.View ArticlePubMedGoogle Scholar
- Neckers L, Ivy SP: Heat shock protein 90. Curr Opin Oncol. 2003, 15: 419-424. 10.1097/00001622-200311000-00003.View ArticlePubMedGoogle Scholar
- Kamal A, Thao L, Sensintaffar J, Zhang L, Boehm MF, Fritz LC, Burrows FJ: A high-affinity conformation of Hsp90 confers tumour selectivity on Hsp90 inhibitors. Nature. 2003, 425: 407-410. 10.1038/nature01913.View ArticlePubMedGoogle Scholar
- Aggarwal BB: Signaling pathways of the TNF superfamily: a double-edged sword. Nat Rev Immunol. 2003, 3: 745-756. 10.1038/nri1184.View ArticlePubMedGoogle Scholar
- Wajant H, Pfizenmaier K, Scheurich P: Tumor necrosis factor signaling. Cell Death Differ. 2003, 10: 45-65. 10.1038/sj.cdd.4401189.View ArticlePubMedGoogle Scholar
- Kelliher MA, Grimm S, Ishida Y, Kuo F, Stanger BZ, Leder P: The death domain kinase RIP mediates the TNF-induced NF-nB signal. Immunity. 1998, 8: 297-303. 10.1016/S1074-7613(00)80535-X.View ArticlePubMedGoogle Scholar
- Ting AT, Pimentel-Muinos FX, Seed B: RIP mediates tumor necrosis factor receptor 1 activation of NF-nB but not Fas/APO-1-initiated apoptosis. EMBO J. 1996, 15: 6189-6196.PubMed CentralPubMedGoogle Scholar
- Zhang J, Winoto A: A mouse Fas-associated protein with homology to the human Mort1/FADD protein is essential for Fas-induced apoptosis. Mol Cell Biol. 1996, 16: 2756-2763.PubMed CentralView ArticlePubMedGoogle Scholar
- Karin M, Yamamoto Y, Wang QM: The IKK NF-κB system: a treasure trove for drug development. Nat Rev Drug Discov. 2004, 3: 17-26. 10.1038/nrd1279.View ArticlePubMedGoogle Scholar
- Yang J, Lin Y, Guo Z: The essential role of MEKK3 in TNF-induced NFκB activation. Nat Immunol. 2001, 2: 620-624.View ArticlePubMedGoogle Scholar
- Wu MX, Ao Z, Prasad KV, Wu R, Schlossman SF: IEX-1L, an apoptosis inhibitor involved in NF-κB-mediated cell survival. Science. 1998, 281: 998-1001.View ArticlePubMedGoogle Scholar
- Wang CY, Mayo MW, Korneluk RG, Goeddel DV, Baldwin AS: NFκB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation. Science. 1998, 281: 1680-1683.View ArticlePubMedGoogle Scholar
- Kamata H, Honda S, Maeda S, Chang L, Hirata H, Karin M: Reactive oxygen species promote TNFa induced death and sustained JNK activation by inhibiting MAP kinase phosphatases. Cell. 2005, 120: 649-661. 10.1016/j.cell.2004.12.041.View ArticlePubMedGoogle Scholar
- Broemer M, Krappmann D, Scheidereit C: Requirement of Hsp90 activity for IκB kinase (IKK) biosynthesis and for constitutive and inducible IKK and NF-κB activation. Oncogene. 2004, 23: 5378-5386. 10.1038/sj.onc.1207705.View ArticlePubMedGoogle Scholar
- Lewis J, Devin A, Miller A: Disruption of hsp90 function results in degradation of the death domain kinase, receptor-interacting protein (RIP), and blockage of tumor necrosis factor-induced nuclear factor-κB activation. J Biol Chem. 2000, 275: 10519-10526. 10.1074/jbc.275.14.10519.View ArticlePubMedGoogle Scholar
- Fukumoto R, Kiang JG: Geldanamycin analog 17-DMAG limits apoptosis in human peripheral blood cells by inhibition of p53 activation and its interaction with heat-shock protein 90 kDa after exposure to ionizing radiation. Radiat Res. 2011, 176: 333-345. 10.1667/RR2534.1.PubMed CentralView ArticlePubMedGoogle Scholar
- Lancet JE, Gojo I, Burton M: Phase I study of the heat shock protein 90 inhibitor alvespimycin (KOS-1022, 17-DMAG) administered intravenously twice weekly to patients with acute myeloid leukemia. Leukemia. 2010, 24: 699-705. 10.1038/leu.2009.292.View ArticlePubMedGoogle Scholar
- Jhaveri K, Miller K, Rosen L: A Phase I Dose-Escalation Trial of Trastuzumab and Alvespimycin Hydrochloride (KOS-1022; 17 DMAG) in the Treatment of Advanced Solid Tumors. Clin Cancer Res. 2012, Epub ahead of printGoogle Scholar
- Palacios C, Martín-Pérez R, López-Pérez AI, Pandiella A, López-Rivas A: Autophagy inhibition sensitizes multiple myeloma cells to 17-dimethylaminoethylamino-17-demethoxygeldanamycin-induced apoptosis. Leuk Res. 2010, 34: 1533-1538. 10.1016/j.leukres.2010.07.002.View ArticlePubMedGoogle Scholar
- Zheng N, Zou P, Wang S, Sun D: In vitro metabolism of 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin in human liver microsomes. Drug Metab Dispos. 2011, 39: 627-635. 10.1124/dmd.110.036418.PubMed CentralView ArticlePubMedGoogle Scholar
- Borkovich KA, Farrelly FW, Finkelstein DB, Taulien J, Lindquist S: Hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures. Mol Cell Biol. 1989, 9: 3919-3930.PubMed CentralView ArticlePubMedGoogle Scholar
- Akner G, Mossberg K, Sundqvist KG, Gustafsson JA, Wikstrom AC: Evidence for reversible, non-microtubule and non-microfilament-dependent nuclear translocation of hsp90 after heat shock in human fibroblasts. Eur J Cell Biol. 1992, 58: 356-364.PubMedGoogle Scholar
- Biggiogera M, Tanguay RM, Marin R, Wu Y, Martin TE, Fakan S: Localization of heat shock proteins in mouse male germ cells: an immunoelectron microscopical study. Exp Cell Res. 1996, 229: 77-85. 10.1006/excr.1996.0345.View ArticlePubMedGoogle Scholar
- Langer T, Rosmus S, Fasold H: Intracellular localization of the 90 kDA heat shock protein (HSP90α) determined by expression of a EGFP-HSP90α-fusion protein in unstressed and heat stressed 3T3 cells. Cell Biol Int. 2003, 27: 47-52. 10.1016/S1065-6995(02)00256-1.View ArticlePubMedGoogle Scholar
- Pratt WB, Toft DO: Steroid receptor interactions with heat shock protein and immunophilin chaperones. Endocr Rev. 1997, 18: 306-360. 10.1210/er.18.3.306.PubMedGoogle Scholar
- Pearl LH, Prodromou C: Structure and mechanism of the Hsp90 molecular chaperone machinery. Annu Rev Biochem. 2006, 75: 271-294. 10.1146/annurev.biochem.75.103004.142738.View ArticlePubMedGoogle Scholar
- Whitesell L, Lindquist SL: HSP90 and the chaperoning of cancer. Nature Rev Cancer. 2005, 5: 761-772. 10.1038/nrc1716.View ArticleGoogle Scholar
- Keane MM, Rubinstein Y, Cuello M: Inhibition of NF-nB activity enhances TRAIL mediated apoptosis in breast cancer cell lines. Breast Cancer Res Treat. 2000, 64: 211-219. 10.1023/A:1006458407515.View ArticlePubMedGoogle Scholar
- Ortiz CM, Ito T, Hashimoto Y, Nagayama S, Iwai A, Tsunoda S: Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines. Diagn Pathol. 2010, 5: 41-10.1186/1746-1596-5-41.PubMed CentralView ArticlePubMedGoogle Scholar
- Lal A, Panos R, Marjanovic M, Walker M, Fuentes E, Kubicek GJ: A gene expression profile test to resolve head & neck squamous versus lung squamous cancers. Diagn Pathol. 2013, 8: 44-10.1186/1746-1596-8-44.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.