Open Access

Primary paranasal sinus hyalinizing clear cell carcinoma: a case report

  • Batool M. AlAli1,
  • Mohammed J. Alyousef1, 2Email author,
  • Ahmad Salah Kamel1, 2,
  • Mohammad A. Al hamad1, 2,
  • Mohammad H. Al-Bar2, 3 and
  • Roaa M. Algowiez2, 4
Diagnostic Pathology201712:70

https://doi.org/10.1186/s13000-017-0659-7

Received: 4 April 2017

Accepted: 14 September 2017

Published: 25 September 2017

Abstract

Background

Hyalinizing clear cell carcinoma (HCCC) is a rare low-grade tumour of salivary glands that was first described as a distinct entity in 1994 by Milchgrub et al. EWSR1-ATF1 fusion was found to be specific for this tumour. The majority of the reported cases of HCCC arise from minor salivary glands within the oral cavity. Primary HCCC of the paranasal sinus is extremely uncommon. To our knowledge, only three cases have been reported in the English literature. Herein, we present a case of HCCC of the posterior ethmoid/maxillary sinus.

Case presentation

A 63-year-old lady who presented with a long history of epistaxis. CT scan revealed a destructive mass in the left ethmoid/posterior maxillary sinus extending to the nasal cavity. Surgical excision was done and microscopic evaluation showed a tumour composed mainly of nests of clear epithelial cells separated by fibrocellular and hyalinized septa with extensive bone destruction. The tumour cells expressed CK5/6, EMA and p63 immunohistochemically but were negative for S100 protein, PAX-8, RCC and CK7. Sinonasal renal cell–like adenocarcinomas, myoepithelial carcinoma and metastatic renal cell carcinoma were excluded by radiological and immunohistochemical studies. Fluorescence in situ hybridization analysis revealed an EWSR1 gene rearrangement. Postoperative radiation was administrated and the patient did not show recurrence or distant metastasis 4 months after the surgery.

Conclusion

Head and neck region have many tumours that demonstrate clear cell changes on histology. Thus, the differential diagnosis for HCCC is wide. Awareness of this rare entity and the possibility of it is arising in unusual location is necessary. EWSR1-AFT1 fusion, a consistent finding in HCCC, can be used to confirm the diagnosis.

Keywords

Hyalinizing clear cell carcinoma Paranasal sinuses EWSR1-ATF1 Salivary gland Clear cell carcinoma

Background

Hyalinizing clear cell carcinoma (HCCC), which is classified by the World Health Organization (WHO) as “Clear cell carcinoma, NOS” [1], is a rare low-grade salivary gland neoplasm with characteristic clear cells arranged in nests and cords embedded within a hyalinizing stroma. Most of the reported cases of HCCC arise from the minor salivary glands within the oral cavity, with base of the tongue and palate being the most common sites [2, 3]. HCCC of paranasal sinuses is an extremely rare tumour, and up to the best of our knowledge, only three cases have been reported in the English literature [4, 5] (Table 1). Herein, we report a case of HCCC arising primarily in paranasal sinuses in a 63-year-old woman. The diagnosis was molecularly confirmed with EWSR1 gene rearrangement.
Table 1

Reported cases of paranasal sinus HCCC

Author

Location

Age (Years)

Gender

EWSR/Rearrangement

Treatment

Follow up

Davina Stasia Teo et al. (2015) [4]

Right ethmoid sinus extending into the nasal cavity.

69

Male

Unknown

Surgical excision

No recurrence after 10 months

Jui Lan et al. (2017) [5]

Right maxillary sinus.

48

Male

Positive

Surgical excision

No recurrence after 9 months

Jui Lan et al. (2017) [5]

Right maxillary sinus.

80

Male

Positive

Surgical excision + radiotherapy

Not provided

Present case

Left ethmoid/posterior maxillary sinuses.

63

Female

Positive

Surgical excision + radiotherapy

No recurrence after 4 months

Case presentation

A 63-year-old lady was referred from ER to the ENT department, complaining of recurrent epistaxis episodes for the past 4 years. She also has history of left sided nasal obstruction, facial pressure, yellowish nasal discharge, protrusion of left eye and anosmia. She gave history of weight loss of around 7 kg in the past 3 months associated with decrease of appetite.

Bilateral nasal endoscopic examination showed a mass that easily bleeds on touch, filling the left nasal cavity and pushing the nasal septum to the other side.

Computed tomography scan (CT) revealed a destructive mass in the left ethmoid/posterior maxillary and sphenoid sinus extending to the nasal cavity. It measured 4 × 4.5 × 6 cm, with extension posteriorly to involve the anterior superior border of the clivus, invading towards the pterygoid bone and reaching the carotid canal (Fig. 1a and b). MRI showed dural thickening lateral to the optic nerve on the left side but no clear intracranial neither periorbital extension. CT scan of chest, abdomen and pelvis were unremarkable for primary or metastatic tumour.
Fig. 1

Head CT scan. Coronal head CT scan showing a destructive lesion within the paranasal sinuses extending to the nasal cavity. The epicenter of the mass is seen within the left ethmoid/posterior maxillary and sphenoid sinus (a) with extension posteriorly to involve the anterior superior border of the clivus (b)

The tumour was treated with surgical excision; however, complete removal was not possible due to attachment to the optic nerve and carotid sheath. Post-operative radiotherapy was administrated.

Multiple soft/tan and bone fragments were received for histological examination. Frozen section was performed for diagnosis and evaluation of margin status. The frozen section diagnosis was reported as “Malignant epithelial neoplasm with prominent clear cell change”. The tumour was extending to the resection margins.

Resected tissues were then fixed in 10% buffered formalin and embedded in paraffin. Permanent hematoxylin and eosin (H&E) sections revealed an infiltrative tumour with extensive bone destruction. The tumour consisted of polygonal to round cells arranged in nests and separated by fibrocellular and hyalinized fibrous septa. Most of the cells had a clear cytoplasm while few cells exhibited eosinophilic cytoplasm especially at the periphery of the tumour. The nuclei were round, uniform and centrally located with inconspicuous nucleoli. Mitotic figures and necrosis were not present. Perineural and lymph-vascular invasion were noted (Fig. 2a-c).
Fig. 2

Histological features of HCCC and FISH analysis. a - c, scanning magnification showing an infiltrative clear cell tumour associated with bone destruction (a, H&E, original magnification 40×), these polygonal to round clear cells are arranged in nests separated by hyalinized fibrocellular septa. Other smaller cells with eosinophilic cytoplasm are seen mostly at the periphery of the tumour (b, hematoxylin and eosin, original magnification 200×). Nuclei are round and central (c, hematoxylin and eosin, original magnification 200×). Diffuse cytoplasmic and membranous staining for CK5/6 (d, immunohistochemistry, original magnification 200×). FISH analysis using dual-color, EWSR1 break apart probe. The tumour cells show one green and one red signal, indicative of a rearrangement of EWSR1 region (e)

The differential diagnosis based on the H&E included squamous cell carcinoma with clear cell changes, mucoepidermoid carcinoma with prominent clear cell change, metastatic renal cell carcinoma and HCCC.

Immunohistochemically, the tumour cells stained positive for EMA, CK5/6 (Fig. 2d), CEA and p63 and were negative for PAX-8, RCC, CK7, SMA and S-100 protein.

FISH analysis of EWSR1 breakapart probe on paraffin-embedded tumour tissue showed evidence of a 22q12 rearrangement in 164 out of 200 (82%) of interphase nuclei scored (Fig. 2e).

The histological, immunohistochemical and molecular findings were consistent with primary hyalinizing clear cell carcinoma of the paranasal sinuses.

On follow-up, the patient showed no evidence of disease 4 months after the surgery.

Discussion and conclusions

HCCC is an uncommon salivary gland neoplasm that is slightly predominant in women (female to male ratio = 1.6 to 1) in their seventh (26%) or sixth (20%) decade of life, with the palate and the base of the tongue being the most common sites of the tumour [2]. Occurrence in paranasal sinuses is very rare. Up to our knowledge, only 3 cases in paranasal sinuses have been reported in the English literature.

The clinical presentation varies according to the location of the tumour, and as the most common location of HCCC is the oral cavity, 74.4% of patients presented with a painless submucosal lump in the oral cavity. Mucosal ulcers, pain, dysphagia and nasal obstruction were also reported [2]. In the present case, the patient presented with epistaxis.

HCCC was first described as a distinct entity by Milchgrub et al. [6] in a series of 11 patients with a distinct salivary gland neoplasm that it is characterized histologically by clear cells arranged in cords, trabeculae and nests with distinct cell borders and small bland looking nuclei. The clear cytoplasm was attributed to its glycogen content rather than mucin. Another smaller population of cells with pale eosinophilic cytoplasm and larger nuclei is seen admixed with the clear cell population. In the present case, such cells were mostly clustered at the periphery of the tumour cell nests. Hyalinizing or fibrocellular stroma separating the neoplastic cells is seen in the majority of cases while mitotic figures are usually rare [6, 7]. Lymph-vascular and perineural invasion is seen in 9.2% and 38.3% of the cases, respectively [2]. These findings were present in the current case.

Immunohistochemically, HCCC tumour cells show immunoreactivity for cytokeratins (CK5, CK7, CK8, CK14 and CK19), p63, epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA) while they are negative for smooth-muscle actin (SMA), muscle-specific actin (MSA), calponin and GFAP. The Ki-67 proliferation index is usually low [2]. PAS stains positive while mucicarmine and PAS-D are negative. This immunohistochemical profile differentiates HCCC from other primary malignancies and metastatic carcinoma.

Sinonasal cancers account for only 3% of all head and neck cancers, with the most common type being squamous cell carcinoma [8, 9]. Sinonasal HCCC has to be differentiated from other more common neoplasms in this region with clear cell changes including: squamous cell carcinoma, sinonasal renal cell–like adenocarcinomas, mucoepidermoid carcinoma, epithelial-myoepithelial carcinoma, myoepithelial carcinoma, acinic cell carcinoma and metastatic carcinoma; particularly, metastatic renal cell carcinoma which is one of the tumours that frequently metastasize to the sinonasal tract [10].

In 2011, Antonescu et al. concluded that EWSR1-ATF1 fusion is the most reliable tool to differentiate HCCC from other histologically similar tumours [11]. In a review of HCCC by Daniele and his coworkers, EWSR1-ATF1 fusion gene rearrangment was confirmed in 43 out of 45 cases. This shows the high sensitivity of this test. However, this translocation is not specific for HCCC; as it is also seen in other malignancies including clear cell sarcoma, angiomatoid fibrous histiocytoma and clear cell odontogenic carcinoma (CCOC). The latter is a recently described entity that is identical in histology and immunohistochemical profile to HCCC. In a study of 12 cases of CCOC by Bilodeau et al., they suggest that the location is the most important distinguishing criterion for these tumours, with CCOC regarded as the central or intraosseous counterpart of HCCC [12, 13]. Mucoepidermoid carcinoma, on the other hand, frequently harbor a translocation t(11;19)(q21;p13) resulting in MECT1-MAML2 fusion. This gene fusion, associated with favourable prognosis, can help in the differential diagnosis [14].

The modality of choice for treatment of HCCC is complete surgical excision with clear margins. The role of post-operative radiotherapy is still not yet proven [2]. Assessment of regional lymph nodes for metastasis is recommended [15].

Even though that this tumour is designated as low-grade, many cases were reported with lymph node, lung and even vertebral metastasis [1618]. Histological features that may suggest a high-grade tumour include: atypical mitosis, necrosis and bizarre neoplastic cells [17, 19]. Patient follow up is recommended even if the tumour is completely excised.

In summary, we report a case of HCCC involving paranasal sinuses primarily and describe its clinical, histopathologic, immunophenotypic, and molecular cytogenetic features. Tumour cell positivity for p63, CK5/6, PAS and negativity for PAX-8, RCC, mucicarmine and PAS-D exclude mucoepidermoid carcinoma, tumours of myoepithelial origin and metastatic renal cell carcinoma.

Abbreviations

CCOC: 

Clear cell odontogenic carcinoma

CEA: 

Carcinoembryonic antigen

CT: 

Computed tomography

EMA: 

Epithelial membrane antigen

ENT: 

Ear, nose and throat clinic

ER: 

Emergency room

HCCC: 

Hyalinizing clear cell carcinoma

NOS: 

Not otherwise specified

SMA: 

Smooth muscle actin

WHO: 

World health organization

Declarations

Acknowledgements

Not applicable.

Funding

The authors declare that this paper was not financially supported.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Authors’ contributions

BA made substantial contributions to conception and design and interpretation of data in addition to drafting the manuscript. MJ was involved in drafting and reviewing the manuscript. AS was involved in reviewing the manuscript and gave the final approval for publication. MH was involved in FISH analysis and interpretation and drafting the FISH cytogenetic part. MB was involved in patient acquisition of data and drafting of the case report part. RG was involved in obtaining radiology pictures with interpretation and drafting of the radiology findings. All authors read and approved the final manuscript.

Authors’ information

Not applicable.

Ethics approval and consent to participate

Not applicable.

Consent for publication

Written informed consent for publication was obtained from the patient.

Competing interests

The authors declare that they have no competing interests.

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Authors’ Affiliations

(1)
Department of Pathology and Laboratory Medicine, King Fahd Hospital of University
(2)
College of Medicine, University of Dammam
(3)
Department of Otolaryngology, King Fahd Hospital of University
(4)
Department of Radiology, King Fahd Hospital of University

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© The Author(s). 2017

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